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| Status |
Public on Sep 15, 2024 |
| Title |
The lifespan and kinetics of human Dendritic Cell subsets and their precursors in health and inflammation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Dendritic cells (DC) are specialised mononuclear phagocytes connecting innate and adaptive immunity. They comprise two principal subsets: plasmacytoid DC and conventional DC. Here, single cell RNA sequencing (scRNA-seq) was performed to analyze the expression levels of different genes in circulating human DC subsets.
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| Overall design |
Following myeloid HLA+ cell enrichment of whole blood according to the method outlined (Lubin et al., 2023). The cells were subsequently stained and sorted using a Fluoresecence-avtivated cell soritng (FACS) to isolate DC. Sorted cells were separated into droplet emulsion using Chromium X (10X Genomics) and analyzed using scRNAseq.
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| Contributor(s) |
Lubin R, Patel AA, Mackerodt J, Zhang Y, Gvili R, Mulder K, Dutertre C, Jalali P, Glanville J, Hazan I, Sridharan N, Kandel L, Rivkin G, Akarca A, Gilroy DW, Marafioti T, Mildner A, Wilensky A, Asquith B, Ginhoux F, Macallan D, Nasereddin A, Shiff I, Yona S |
| Citation missing |
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| Submission date |
Sep 06, 2024 |
| Last update date |
Sep 16, 2024 |
| Contact name |
Ruth Lubin |
| E-mail(s) |
ruti.lubin@mail.huji.ac.il
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| Organization name |
Hebrew university
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| Department |
The Institute of Biomedical and Oral Research
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| Lab |
Dr. Simon Yona, Mononuclear Phagocyte Immunobiology Group
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| Street address |
Ein-Kerem
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| City |
Jerusalem |
| ZIP/Postal code |
9112102 |
| Country |
Israel |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA1157768 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE276518_RAW.tar |
46.3 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
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