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| Status |
Public on Sep 10, 2024 |
| Title |
Effect of glutamine and alphaketoglutarate in gene expression |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Glutamine (gln) is an essential and versatile metabolite that feeds different cellular pathways and generates several derivatives. Alpha-ketoglutarate (aKG) is the gln derivative that feeds the TCA cycle, activates cellular growth, and is related to epigenetic changes. Despite gln importance, the specific role of gln – and not its derivatives – in gene expression is not clear. To address this question, U2OS cells were cultured in the presence of gln or its absence +/- aKG for 8 hours. Gln had an unique effect in the transcriptome of U2OS cells and the addition of aKG was not able to mimic the gln effect. In conclusion, gln affects gene expression independently of its derivative aKG.
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| Overall design |
To assess the role of gln in regulation gene expression, U2OS cells were cultured in the presence of gln or its absence +/- aKG for 8 hours.
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| Contributor(s) |
Martins Garcia B, Mederios T, Pernas L |
| Citation missing |
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| |
| Submission date |
Sep 04, 2024 |
| Last update date |
Sep 10, 2024 |
| Contact name |
Bruna Martins Garcia |
| E-mail(s) |
brunamgarcia@yahoo.com.br
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| Organization name |
Max Planck Institute for Biology of Ageing
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| Street address |
Joseph-Stelzmann-Strasse 9B
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| City |
Cologne |
| ZIP/Postal code |
50931 |
| Country |
Germany |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA1156636 |
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