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| Status |
Public on Aug 22, 2024 |
| Title |
B7-H3-Targeted CAR-Vδ1T Cells Exhibit Potent Broad-Spectrum Activity Against Solid Tumors |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Vδ1T cells, a rare subset of γδT cells, hold promise for treating solid tumors. Unlike conventional T cells, they recognize tumor antigens independently of the MHC antigen-presentation pathway, making them a potential “off-the-shelf” cell therapy product. However, isolation and activation of Vδ1T cells is challenging, which has limited their clinical investigation. Here, we developed a large-scale clinical-grade manufacturing process for Vδ1T cells and validated the therapeutic potential of B7-H3-CAR-modified Vδ1T cells in treating solid tumors. Co-expression of interleukin-2 with the B7-H3-CAR led to durable anti-tumor activity of Vδ1T cells in vitro and in vivo. In multiple subcutaneous and orthotopic mouse xenograft tumor models, a single intravenous administration of the CAR-Vδ1T cells resulted in complete tumor regression. These modified cells demonstrated significant in vivo expansion and robust homing ability to tumors, akin to natural tissue-resident immune cells.Additionally, the B7-H3-CAR-Vδ1T cells exhibited a favorable safety profile. In conclusion, B7-H3-CAR-modified Vδ1T cells represent a promising strategy for treating solid tumors.
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| Overall design |
To investigate the molecular mechanisms underlying the superior efficacy of IL-2-expressing AQ-CAR-IL2-Vδ1T cells compared to AG-CAR-Vδ1T cells, we performed single-cell RNA sequencing on cells after repeated antigen stimulation with Raji-B7-H3 cells in vitro for four consecutive days.
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| Contributor(s) |
Yang L, Jiang L, You F |
| Citation missing |
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| Submission date |
Aug 20, 2024 |
| Last update date |
Aug 22, 2024 |
| Contact name |
Licui Jiang |
| E-mail(s) |
licui.jiang@persongen.com
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| Organization name |
Soochow University
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| Street address |
Dushuhu Campus, Soochow University, No. 199, Ren'ai Road/Street
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| City |
Suzhou |
| State/province |
None Selected |
| ZIP/Postal code |
215123 |
| Country |
China |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA1150094 |
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