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Series GSE27524 Query DataSets for GSE27524
Status Public on Jul 01, 2013
Title A functional liaison between E2F and the aberrant ETS oncogene EWS/FLI1 in Ewing's sarcoma
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Translocations of ETS transcription factors are driver mutations in diverse cancers. We investigated the genomic network of the ETS fusion EWS/FLI1 in Ewing's sarcoma (ESFT) as a model of ETS-driven tumorigenesis. ChIP-Seq and transcriptional analysis identified E2F3 as a principle co-factor of EWSFLI1 defining functionally distinct gene sets. While EWS/FLI1 binding independent of E2F3 predominantly associated with repressed differentiation genes, significant co-localization with E2F3 was discovered at proximal promoters of activated growth-related genes. Thus, EWS/FLI1 promotes oncogenesis by simultaneously perturbing differentiation state and augmenting the expression of genes co-regulated by E2F3. Integration of additional E2F3 and ERG localization data from prostate cancer containing TMPRSS2/ERG verified that the ETS-E2F module is also found in prostate cancer and may be of general relevance to ETS driven cancers.
Overall design Timecourse with 6 timepoints of a doxicyclin inducible EWS-FLI1 knockdown in the A673 Ewing's Sarcoma celline
Contributor(s) Bilke S, Schwentner R, Yang F, Kauer M, Walker RL, Kovar H, Meltzer PS
Citation(s) 23940108
Submission date Feb 25, 2011
Last update date Dec 06, 2018
Contact name Maximilian Kauer
Organization name CCRI, St.Anna Children Cancer Research Institute
Department Molecular Biology
Lab Heinrich Kovar
Street address Zimmermannplatz 10
City Vienna
ZIP/Postal code 1090
Country Austria
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (16)
GSM679847 A673_CloneAsp14_Dox_0h_1
GSM679848 A673_CloneAsp14_Dox_0h_2
GSM679849 A673_CloneAsp14_Dox_0h_3
BioProject PRJNA137111

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE27524_RAW.tar 30.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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