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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Aug 14, 2024 |
| Title |
Axonopathy precedes cell death in ocular damage mediated by blast exposure |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Traumatic brain injuries (TBI) of varied types are common across all populations and can cause visual problems. For military personnel in combat settings, injuries from blast exposures (bTBI) are prevalent and arise from a myriad of different situations. To model these diverse conditions, we are one of several groups modeling bTBI using mice in varying ways. Here, we report a refined analysis of retinal ganglion cell (RGC) damage in male C57BL/6J mice exposed to a blast-wave in an enclosed chamber. Ganglion cell layer thickness, RGC density (BRN3A and RBPMS immunoreactivity), cellular density of ganglion cell layer (hematoxylin and eosin staining), and axon numbers (paraphenylenediamine staining) were quantified at timepoints ranging from 1 to 17-weeks. RNA sequencing was performed at 1-week and 5-weeks post-injury. Earliest indices of damage, evident by 1-week post-injury, are a loss of RGC marker expression, damage to RGC axons, and increase in glial markers expression. Blast exposure caused a loss of RGC somas and axons-with greatest loss occurring by 5-weeks post-injury. While indices of glial involvement are prominent early, they quickly subside as RGCs are lost. The finding that axonopathy precedes soma loss resembles pathology observed in mouse models of glaucoma, suggesting similar mechanisms.
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| Overall design |
We performed gene expression profile analysis on retinas from male C5BL/6J mice exposed to a blast-wave in an enclosed chamber at 1-week and 5-weeks post-injury. The retina was from the eye exposed directly to the blast wave. There were 12 mice at each time-point 6 sham and 6 injured.
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| Contributor(s) |
Boehme NA, Hedberg-Buenz A, Tatro N, Bielecki M, Castonguay WC, Scheetz TE, Anderson MG, Dutca LM |
| Citation(s) |
34083587 |
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| Submission date |
Aug 14, 2024 |
| Last update date |
Aug 14, 2024 |
| Contact name |
Todd Scheetz |
| E-mail(s) |
todd-scheetz@uiowa.edu
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| Organization name |
UNIVERSITY OF IOWA
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| Street address |
3181B MERF
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| City |
Iowa City |
| State/province |
IA |
| ZIP/Postal code |
52242 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA1148067 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE274793_5week_expression.txt.gz |
1.5 Mb |
(ftp)(http) |
TXT |
| GSE274793_8day_expression.txt.gz |
1.4 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
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