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| Status |
Public on Sep 12, 2024 |
| Title |
WT and N88S seipin-expressing cells upon bathophenanthrolinedisulfonate (BPS) treatment |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
WT and N88S seipin-expressing cells were grown in SC-glucose medium to exponential phase and then iron chelator bathophenanthrolinedisulfonate (BPS) was added to a final concentration of 100 micromolar. Cells were incubated in the presence of BPS for an additional 4 hour period. We intend to evaluate differentially expressed genes (DEGs) between the two strains. Four biological replicates (classified as 1-4 for the corresponding strain) were used per strain.
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| Overall design |
Cells were treated with BPS to induce iron starvation conditions and evaluate differentially expressed genes (DEGs): WT (G1, 4 biological replicates) vs N88S (G2, 4 biological replicates).
2024 BGI Genomics Co.,Ltd.
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| Contributor(s) |
Teixeira V, Ribeiro M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Aug 04, 2024 |
| Last update date |
Sep 12, 2024 |
| Contact name |
Vitor Teixeira |
| E-mail(s) |
vitor.fcup@gmail.com
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| Organization name |
i3s
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| Department |
YSN
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| Street address |
Rua Alfredo Allen 208
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| City |
Porto |
| State/province |
Porto |
| ZIP/Postal code |
4200-135 |
| Country |
Portugal |
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| Platforms (1) |
| GPL33987 |
DNBSEQ-T7 (Saccharomyces cerevisiae) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA1144137 |
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