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| Status |
Public on Jul 25, 2024 |
| Title |
Novel Targets for Diabetic Foot Ulcer Healing: A Transcriptomic Analysis by Negative Pressure Wound Therapy |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The results of the present study provide new insights into the mechanism through which NPWT promotes DFU wound healing
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| Overall design |
Participants underwent wound debridement to remove blackened and necrotic soft and bone tissues, followed by NPWT. For NPWT, a vacuum assisted closure (VAC) negative pressure-assisted healing therapy system (KCI, USA) was used according to published protocols , at a dose of -125 mmHg for 1 week. Full-thickness skin tissue was taken within 0.5 cm of the wound edge before and after 1 week of NPWT, only after removing the negative pressure device. The collected granulation tissue was stored at -80ºC for examination.RNA-seq was performed on the granulation tissue of three patients with DFU before and after NPWT (before/after-patient 1, before/after-patient 2, before/after-patient 3)
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| |
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| Citation(s) |
39301661 |
| |
| Submission date |
Jul 23, 2024 |
| Last update date |
Oct 09, 2024 |
| Contact name |
Ying Tang |
| E-mail(s) |
chenmingwei@ahmu.edu.cn
|
| Phone |
+8613855365517
|
| Organization name |
Department of Endocrinology, the First Affiliated Hospital of Anhui Medical University
|
| Street address |
No.218 Jixi Road, Shushan District, Hefei City, Anhui Province, China.
|
| City |
hefei |
| ZIP/Postal code |
230032 |
| Country |
China |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA1139226 |
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