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| Status |
Public on Sep 05, 2024 |
| Title |
Effect of depletion of Drosophila Hepatocyte Nuclear Factor 4 (dHNF4) in enterocytes on gene expression in the adult midgut |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The main objective of the sudy was to characterize the functions of Drosophila Hepatocyte Nuclear Factor 4 (dHNF4) in midgut enterocytes. For this, we expressed a UAS-dHNF4 RNAi transgene under the control of mex-GAL4, which is active specifically in midgut enterocytes. We then collected adult male midguts, extracted total RNA and performed mRNA-seq. Our data demonstrate that dHNF4 acts as a master regulator of intestinal lipid metabolism in Drosophila.
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| Overall design |
Total RNA was extracted from 20 midguts of adult male flies ( controls - genotype: w1118/Y ; ; UAS-dHNF4 RNAi NIG9310-R2/+), and of adut male flies expressing a UAS-dHNF4 RNAi transgene under the control of the enterocyte-specific mex-GAL4 driver (genotype: w/Y ; mex-GAL4/+; UAS-dHNF4 RNAi NIG9310-R2/+). mRNA sequencing was performed on 5 biological replicates per genotype. Adult male flies were fed a yeast only diet and dissected 7 days after eclosion.
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| Web link |
https://www.sciencedirect.com/science/article/pii/S2211124724010441?via%3Dihub
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| Contributor(s) |
Storelli G, Vonolfen MC, Gyenis A |
| Citation(s) |
39235946 |
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| Submission date |
Jul 08, 2024 |
| Last update date |
Sep 10, 2024 |
| Contact name |
Gilles Storelli |
| Organization name |
University of Cologne
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| Department |
CECAD Research Center
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| Lab |
Storelli Lab
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| Street address |
Joseph Stelzmannstrasse 26
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| City |
Cologne |
| ZIP/Postal code |
50931 |
| Country |
Germany |
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| Platforms (1) |
| GPL25244 |
Illumina NovaSeq 6000 (Drosophila melanogaster) |
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| Samples (10)
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| GSM8383995 |
Adult Midgut, Control, sample#4 |
| GSM8383996 |
Adult Midgut, Control, sample#5 |
| GSM8383997 |
Adult Midgut, dHNF4 RNAi, sample#1 |
| GSM8383998 |
Adult Midgut, dHNF4 RNAi, sample#2 |
| GSM8383999 |
Adult Midgut, dHNF4 RNAi, sample#3 |
| GSM8384000 |
Adult Midgut, dHNF4 RNAi, sample#4 |
| GSM8384001 |
Adult Midgut, dHNF4 RNAi, sample#5 |
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| Relations |
| BioProject |
PRJNA1133025 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE271746_resultsRUVSeq_mex_HNF4_RNAI.xlsx |
6.2 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
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