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| Status |
Public on Jul 04, 2024 |
| Title |
Transcriptome Analysis of the Effects of High Temperature on Zygotic Genome Activation in Porcine Embryos |
| Organism |
Sus scrofa |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Damage to the development of porcine gametes and embryos caused by high temperatures (HT) is one of the main reasons for the decline in the economic benefits of the livestock industry. Zygotic genome activation (ZGA) marks the beginning of gene expression programs in mammalian pre-implantation embryos. In pigs, ZGA occurs at the 4-cell (4C) stage, indicating that correct gene expression at this stage plays an important regulatory role in embryonic development. However, the effect of the HT environment on early porcine embryonic development and the RNA expression profile of ZGA remain unclear. In this study, we compared the RNA transcription patterns of porcine 4C embryos under normal and HT conditions using RNA-seq and identified 326 differentially expressed genes (DEGs). These changes were mainly related to DNA polymerase activity, DNA replication, and nucleotidyltransferase activity. In addition, entries for reverse transcription and endonuclease activity were enriched, indicating that ZGA interfered under HT conditions. Further comparison of the experimental results with the porcine ZGA gene revealed 39 ZGA genes among the DEGs. KEGG and GSEA analysis showed that the oxidative phosphorylation pathway was significantly enriched and signaling pathways related to energy metabolism were significantly downregulated. We also found that NDUFA6 and CDKN1A were located at the center of the protein-protein interaction network diagram of the DEGs. In summary, HT conditions affect mitochondrial function and oxidative phosphorylation levels, and lead to changes in the expression pattern of ZGA in early porcine embryos, with its hub genes NDUFA6 and CDKN1A.
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| Overall design |
To explore the effect of high temperature on zygotic genome activation of porcine embryos, the embryos were cultured at 41.5 ℃. Embryos were collected at the 4-cell stage for sequencing.
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| Web link |
https://www.nature.com/articles/s41598-024-73166-5
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| Contributor(s) |
Sun M, Zhan C, Li X, Lee S, Cui X |
| Citation(s) |
39300156 |
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| Submission date |
Jul 01, 2024 |
| Last update date |
Oct 07, 2024 |
| Contact name |
Chenglin Zhan |
| E-mail(s) |
kinglingggg@gmail.com
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| Organization name |
chungbuk national university
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| Department |
department of animal science
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| Street address |
Chungdae-ro 1, Seowon-Gu
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| City |
Cheongju |
| State/province |
Chungcheong-buk do |
| ZIP/Postal code |
28644 |
| Country |
South Korea |
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| Platforms (1) |
| GPL26351 |
Illumina NovaSeq 6000 (Sus scrofa) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA1130363 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE271158_Expression_Profile.txt.gz |
2.5 Mb |
(ftp)(http) |
TXT |
| GSE271158_RAW.tar |
3.9 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
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