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| Status |
Public on Jul 08, 2024 |
| Title |
in vitro construction of an embryonic caudal organizer |
| Organisms |
Danio rerio; Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: Characterization of cell types in bmp4 explants and BMP4 induced human embryoids. Methods: Single cell transcriptomes of bmp4 explants were harvested at 6 hpf, 8 hpf,10 hpf, 12 hpf, 18 hpf and 24 hpf. Single cell transcriptomes of BMP4 induced human embryoids were harvested at Day 5 and Day 6. Libraries were prepared using Single Cell 3′Library & gel Bead kit v3.1 (10x Genomics, Cat# PN-1000121) according to the manufacturer’s protocol for 10000 cell recovery. Results: paraxial mesoderm, lateral plate mesoderm, and their derivatives were detected in bmp4 explants, and NMP, somite and neural cell fates were detected in bmp4 induced human embryoids. Conclusions: A well-organized caudal-like structure was induced in bmp4 explants and caudal cell fates were specified in BMP4 induced human embryoids.
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| Overall design |
bmp4 injected zebrafish explants or BMP4 induced human embryoids were harvested for scRNA-seq.
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| Contributor(s) |
Cheng T, Xing Y, Huang Y |
| Citation missing |
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| |
| Submission date |
Jun 27, 2024 |
| Last update date |
Jul 08, 2024 |
| Contact name |
Tao cheng |
| E-mail(s) |
11818118@zju.edu.cn
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| Phone |
0571-88208276
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| Organization name |
Zhejiang University
|
| Department |
Institute of genetics
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| Street address |
886 Yuhangtang Road
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| City |
Hang Zhou |
| State/province |
Zhe Jiang |
| ZIP/Postal code |
310000 |
| Country |
China |
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| Platforms (2) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
| GPL24995 |
Illumina NovaSeq 6000 (Danio rerio) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA1129168 |
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