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| Status |
Public on Jun 26, 2024 |
| Title |
Activation of three targets by a TAL effector confers susceptibility to bacterial blight of cotton |
| Organism |
Gossypium hirsutum |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Xanthomonas spp. employ transcription activator-like effectors (TALEs) to promote pathogenicity by activating host susceptibility (S) genes. Cotton GhSWEET10 is an S gene targeted by a TALE in an early isolate of Xanthomonas citri pv. malvacearum (Xcm), but not by recent field Xcm isolates. To understand the pathogenicity shift in Xcm and its adaptation to cotton, we assembled the whole genome and the TALE repertoire of three recent Xcm Texas field isolates. A newly evolved TALE, Tal7b, activated different GhSWEET genes, GhSWEET14a and GhSWEET14b. Simultaneous activation of GhSWEET14a and GhSWEET14b resulted in pronounced water-soaked lesions. Transcriptome profiling coupled with TALE-binding element prediction identified a pectin lyase as an additional Tal7b target, quantitatively contributing to Xcm virulence alongside GhSWEET14a/b. CRISPR-Cas9-based gene editing supported the function of GhSWEETs as S genes in cotton bacterial blight and the promise of disrupting the TALE-binding site in these genes to control the disease. Collectively, our findings elucidate the rapid evolution of TALEs in Xanthomonas field isolates and highlight the virulence mechanism wherein TALEs induce multiple S genes simultaneously to promote pathogenicity.
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| Overall design |
To identify the genes being targeted and upregulated by virulence protein Tal7b from the plant pathogen Xanthomonas citri pv. malvacearum, we expressed Tal7b or an vector control into susceptible cotton variety FM2322. We performed transcriptome profile analysis using data obtained from RNA-seq of 6 different cotton plants inoculated with either Tal7b or vector control Differential gene expression profile analysis of RNA-seq data for cotton plants infected with Xanthomonas expressing Tal7b or vector control (T7b, HM)
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| Citation missing |
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| Submission date |
Jun 24, 2024 |
| Last update date |
Jun 26, 2024 |
| Contact name |
Brendan MORMILE |
| E-mail(s) |
mormileb@umich.edu
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| Organization name |
University of Michigan
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| Street address |
211 redmond drive
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| City |
college station |
| State/province |
Texas |
| ZIP/Postal code |
77840 |
| Country |
USA |
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| Platforms (1) |
| GPL16485 |
Illumina HiSeq 2000 (Gossypium hirsutum) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA1127543 |
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