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| Status |
Public on Jun 29, 2024 |
| Title |
The specificity of hpCasMINI for gene editing [Tag-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Other
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| Summary |
The hpCasMINI exhibits high specificity for gene editing. Compared to SpCas9 and LbCas12a, hpCasMINI also exhibited higher specificity for genomic DNA cleavage at the tested sites.
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| Overall design |
We evaluated the specificity of hpCasMINI-mediated gene editing in human cells using Tag-seq. Within the 10 tested sites, hpCasMINI exhibited about 2.5-fold increased activity and similar specificity compared to CasMINI. we also compared the DNA cleavage activity of SpCas9, LbCas12a, CasMINI, and hpCasMINI across four genomic sites at the same location and five sites at adjacent locations, hpCasMINI also exhibited higher specificity for genomic DNA cleavage at the tested sites.
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| Contributor(s) |
Ma S, Chen K, Lin Y, Rong Z |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jun 24, 2024 |
| Last update date |
Jun 29, 2024 |
| Contact name |
Kechen Chen |
| E-mail(s) |
kechenchen_orcid@126.com
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| Organization name |
Southern Medical University
|
| Street address |
Shatai South Road
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| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510515 |
| Country |
China |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA1127530 |
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