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| Status |
Public on Jun 25, 2024 |
| Title |
Polyopes affinis Suppressed IFN-γ- and TNF-α-Induced Inflammation in Human Keratinocytes via Down-Regulation of the NF-κB and STAT1 Pathways |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Polyopes affinis is a red algal species commonly found on the South coast and near Jeju Island, Korea. This study aimed to determine whether P. affinis extracts can inhibit the pathogenesis of T-helper-2 (Th2)-mediated inflammation in a human keratinocyte cell line of atopic dermatitis (AD). Cells were incubated with 10 ng/mL of interferon gamma (IFN-γ) and 10 ng/mL of tumor necrosis factor-alpha (TNF-α) at various concentrations of PAB (10, 30, and 60 µg/mL) and PAA (100, 500, and 1,000 µg/mL) extracts. A gene-ontology (GO)-enrichment analysis revealed that PAB significantly enriched the genes associated with biological processes such as cell adhesion, immune response, inflammation, and chemokine-mediated pathways. PAB suppressed the ex-pression of the secretory proteins and mRNAs that are associated with the thymus and the production of activation-regulated chemokines (TARC/CCL17) and macrophage-derived chemo-kines (MDC/CCL22). The effect of the extract on mitogen-activated protein kinases (MAPKs) was related to its inhibition of TARC/CCL17 and MDC/CCL22 production by blocking NF-κB and STAT1 activation. These results suggest that seaweed extract may improve AD by regulating pro-inflammatory chemokines. In conclusion, we first confirmed the existence of phloroglucinol, a polyphenol formed from a precursor called phlorotannin, which is present in PAB, and this result proved the possibility of PAB being used as a treatment for AD.
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| Overall design |
To investigate target genes and mechanism related to anti-inflammation by Polyopes affinis extract in HaCaT cell line stimulated with IFN-γ and TNF-α, we performed gene expression profiling analysis using data obtained from RNA-Seq of 3 groups(solvent control group, IFN-γ and TNF-α treated group, Polyopes affinis extract with IFN-γ and TNF-α treated group). Each group was treated with three dishes, and the RNA obtained from each dish was pooled after adjusting the concentration for RNA sequencing.
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| Web link |
http://10.3390/molecules27061836
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| Contributor(s) |
Ha Y, Lee W, Kim J, Jeon H, Lee J, Kim Y |
| Citation(s) |
35335198 |
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| Submission date |
Jun 21, 2024 |
| Last update date |
Jun 25, 2024 |
| Contact name |
Youn-Jung Kim |
| E-mail(s) |
duckyj@inu.ac.kr
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| Organization name |
Incheon National University
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| Street address |
Academyro 119
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| City |
Incheon |
| ZIP/Postal code |
22012 |
| Country |
South Korea |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA1126629 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE270435_RAW.tar |
370.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
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