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| Status |
Public on Jun 25, 2024 |
| Title |
Effect of FL-Fc-DM1 drug on gene expression of THP-1 cells pretreated with cytarabine. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Cytarabine is one of the first line chemo drugs to treat acute myeloid leukemia (AML).
However, cytarabine can induce AML cell cell cycle arrest at G0/G1 phase. These cell-cycle-arrested AML cells resist cytarabine, show a senescence-like phenotype or stem-cell like phenotype.
To eliminate these cell-cycle arrested cells, we developed a FL-Fc-DM1 drug by conjugating FLT3 liangd Fc fusion protein and mertansine. We used the THP-1 cells as a AML model.
The total mRNA of THP-1 cells treated with different drugs (cytarabine, FL-Fc-DM1, FL-Fc) were sequenced.
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| Overall design |
THP-1 cells were cultured in RPMI 1640 medium supplemented with 10% FBS.
In the control group, THP-1 cells were cultured for 4 days. In the cytarabine group, THP-1 cells were treated with 20 µM cytarabine for 4 days. In the FL-Fc-DM1 treatment group, THP-1 cells were treated by cytarabine for 3 days and then treated with FL-Fc-DM1 (5 nM) for another 24 hours in the absence of cytarabine. In the FL-Fc treatment group, THP-1 cells were treated by cytarabine for 3 days and then treated with FL-Fc (5 nM) for another 24 hours in the absence of cytarabine.
We than analyze the transcriptome differences between different groups.
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| Contributor(s) |
Zhang D, Chen Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jun 20, 2024 |
| Last update date |
Jun 25, 2024 |
| Contact name |
Dengyang Zhang |
| E-mail(s) |
epazhang@outlook.com
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| Organization name |
Seventh affiliated hospital, Sun Yat-sen University
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| Department |
Scientific research center
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| Street address |
Zhenmei road, guangming district
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| City |
Shenzhen |
| State/province |
Guangdong |
| ZIP/Postal code |
518107 |
| Country |
China |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA1126229 |
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