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Series GSE2703 Query DataSets for GSE2703
Status Public on Nov 02, 2005
Title Circadian gene expression in the primate adrenal gland
Platform organism Homo sapiens
Sample organism Macaca mulatta
Experiment type Expression profiling by array
Summary Circadian regulation of gene expression in central and peripheral tissue has been studied in mice. The biomedical implications of this findings led us to the development of a model in which to study the circadian mechanisms underlying primate physiology.
The objective of this experiment has been to identify genes that are circadianly regulated in the monkey (macaca mulatta) adrenal gland.
We analyzed the data using three filters: the first one for presence in at least three out of six time points (presence call=P, P value<0.05). The second one for high amplitude oscillations, using the coefficient of variation with a cutt of value of 0.2 (selecting probesets above that value). The last one for circadian expression, calculating the correlation of each curve to cosine curves of 24h periodicity and defined phases with increments of 10 minutes from 0 to 24h.
After two filtrations, the probesets obtained were identified using gene ID, PubMed accession number, and description, and clustered into well-known pathways.
Identification of clock genes was used as an internal control for the determination of phase distribution of the transcripts across the day.
Part of the array data has been validated by sq PCR, real time-PCR and immunohistochemistry, with succesful results.
This study provides evidences for circadian mechanisms of gene expression in primate tissue, in vivo.
Keywords = primate
Keywords = circadian rhythm
Keywords = adrenal gland
Keywords: time-course
Contributor(s) Lemos DR, Downs JL, Urbanski HF
Citation(s) 16439464
Submission date May 24, 2005
Last update date Aug 10, 2018
Contact name Dario Lemos
Phone 503 690 5387
Organization name Oregon National Primate Research Center
Street address
City Beaverton
State/province OR
ZIP/Postal code 97006
Country USA
Platforms (1)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
Samples (6)
GSM52012 Monkey adrenal gland 3 AM
GSM52013 Monkey adrenal gland 7 AM
GSM52014 Monkey adrenal gland 11 AM
BioProject PRJNA92349

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Supplementary file Size Download File type/resource
GSE2703_RAW.tar 20.4 Mb (http)(custom) TAR (of CEL)

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