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| Status |
Public on Sep 19, 2024 |
| Title |
Bulk RNAseq of adult Drosophila melanogaster whole brains with neuronal specific knock down of brummer. |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Lipids are essential for neuron development and physiology. Lipid droplets (LD) play a central role in coordinating cellular lipid distribution and metabolism in non-neuronal cells; however, there is no general consensus on a role for neuronal LD under normal physiological conditions. We have previously shown that neuronal LD exists in wildtype flies under normal physiological condition, and that the loss of a triglyceride lipase, brummer, increases neutral lipids in the brain (Wat et al., 2020). To investigate the importance of brummer in regulating neuronal metabolism and function in a sex-specific manner, we performed transcriptomic profiling in adult male and female whole brain with RNAi-mediated neuron-specific knock down of brummer.
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| Overall design |
To assess the transcriptomic effects of brummer knock down in either males or females, we raised male and female flies with neuron-specific expression of RNAi targeting brummer transcripts (elav-GAL4>UAS-bmm-RNAi). Adult whole brains were dissected at day 5 to 6 after eclosion for transcriptomic profiling. We also profiled the transcriptome of whole brains from elav-GAL4>+ and +>UAS-bmm-RNAi males and females as controls.
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| Contributor(s) |
Rideout E, Miller C, Hollman S, Chao C |
| Citation missing |
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| Submission date |
Jun 17, 2024 |
| Last update date |
Sep 19, 2024 |
| Contact name |
Elizabeth Rideout |
| E-mail(s) |
rideoute@mail.ubc.ca
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| Organization name |
University of British Columbia
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| Department |
Department of Cell and Physiological Science
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| Lab |
Rideout lab
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| Street address |
2350 Health Sciences Mall
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| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V6T 1Z3 |
| Country |
Canada |
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| Platforms (1) |
| GPL30203 |
NextSeq 2000 (Drosophila melanogaster) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA1125027 |
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