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| Status |
Public on Sep 30, 2024 |
| Title |
Generation of Alveolar Assembloids (AlvAssemb) with Functional Alveolar-like Macrophages |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Within the human lung, interplay between alveolar epithelial cells and resident macrophages shape lung development and function in both health and disease. To study these processes, we devised a strategy for co-culturing hPSC-derived alveolar epithelial organoids and induced macrophages to form functional environment, which we name alveolar assembloids (AlvAssemb). By scRNAseq and functional analysis of AlvAssemb, we identified alveolar type 2-like cells that produced GM-CSF, a response associated with tissue adaptation of macrophages, and that resident alveolar macrophage-like cells secreted interleukin-1β and interleukin-6, a core immune response function, and expressed genes required for surfactant metabolism. Remarkably, in response to alveolar epithelial injury, we found that macrophage-like cells within AlvAssemb efficiently eliminated damaged cells and absorbed oxidized lipids. Moreover, in response to lipopolysaccharide exposure and Mycobacterium tuberculosis infection, we found that core features of the human respiratory defense response were replicated in AlvAssemb. These findings support that AlvAssemb could serve as a valuable platform to investigate human lung development and pathology.
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| Overall design |
We cultured iMφ with and without GM-CSF (10 ng/ml) for 7 days. The cells were then infected with M.tb (H37Rv) at MOIs of 5 and 10 for 48 hours. Following infection, RNA sequencing libraries were prepared and the results were analyzed using RNA-seq.
Please note that GSM4106642 (Ni, huPrimary_0_r1) and GSM4106641 (Moi-20, huPrimary_20) data (in GSE138398) was re-analyzed in the current study.
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| Contributor(s) |
Gil D, Lee Y, Lee M, Kim J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jun 13, 2024 |
| Last update date |
Sep 30, 2024 |
| Contact name |
Dayeon Gil |
| E-mail(s) |
gilje@korea.kr
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| Phone |
+82-43-249-2527
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| Organization name |
Korea national institute of health
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| Department |
Division of intractable disease research
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| Street address |
202 Osongsaengmyung 2-ro, Heungdeok-gu
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| City |
Cheongju-si |
| State/province |
Chungcheongbuk-do |
| ZIP/Postal code |
28160 |
| Country |
South Korea |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (12)
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| GSM8325882 |
iMAC infected M.tb (H37Rv) at MOIs of 10,RNAseq [0_10_2] |
| GSM8325883 |
iMAC infected M.tb (H37Rv) at MOIs of 5,RNAseq [0_5_1] |
| GSM8325884 |
iMAC infected M.tb (H37Rv) at MOIs of 5,RNAseq [0_5_2] |
| GSM8325885 |
iMAC with GM-CSF,RNAseq [GM_0_1] |
| GSM8325886 |
iMAC with GM-CSF infected ,RNAseq [GM_0_2] |
| GSM8325887 |
iMAC with GM-CSF infected M.tb (H37Rv) at MOIs of 10,RNAseq [GM_10_1] |
| GSM8325888 |
iMAC with GM-CSF infected M.tb (H37Rv) at MOIs of 10,RNAseq [GM_10_2] |
| GSM8325889 |
iMAC with GM-CSF infected M.tb (H37Rv) at MOIs of 5,RNAseq [GM_5_1] |
| GSM8325890 |
iMAC with GM-CSF infected M.tb (H37Rv) at MOIs of 5,RNAseq [GM_5_2] |
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| Relations |
| Reanalysis of |
GSM4106642 |
| Reanalysis of |
GSM4106641 |
| BioProject |
PRJNA1123576 |
| Data table header descriptions |
| GEO Sample |
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| GEO Series |
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| SRA Experiment |
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| BioSample |
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| BioProject |
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| Sample title |
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| Data table |
| GEO Sample |
GEO Series |
SRA Experiment |
BioSample |
BioProject |
Sample title |
| GSM4106642 |
GSE138398 |
SRX6947212 |
SAMN12908435 |
PRJNA575728 |
Ni |
| GSM4106641 |
GSE138398 |
SRX6947211 |
SAMN12908436 |
PRJNA575728 |
Moi-20 |
Total number of rows: 2
| Supplementary file |
Size |
Download |
File type/resource |
| GSE269733_rsem.merged.gene_counts.tsv.gz |
979.1 Kb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
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