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| Status |
Public on Jun 09, 2024 |
| Title |
Humanized CXCL12 Antibody Delays Onset and Modulates Immune Response in Alopecia Areata Mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
It has been demonstrated that CXCL12 inhibits hair growth via CXCR4, and its neutralizing antibody (Ab) increases hair growth in alopecia areata (AA). However, the molecular mechanisms have not been fully elucidated. In the present study, we further prepared humanized CXCL12 Ab for AA treatment and investigated underlying molecular mechanisms using single-cell RNA sequencing. Subcutaneous injection of humanized CXCL12 Ab significantly delayed AA onset in mice, and dorsal skin was analyzed. T cells and dendritic cells/macrophages were increased in the AA model, but decreased after CXCL12 Ab treatment. Pseudobulk RNA sequencing identified 153 differentially expressed genes that were upregulated in AA model and downregulated after Ab treatment. Gene ontology analysis revealed that immune cell chemotaxis and cellular response to type II interferon were upregulated in AA model but downregulated after Ab treatment. We further identified key immune cell-related genes such as Ifng, Cd8a, Ccr5, Ccl4, Ccl5, and Il21r, which were colocalized with Cxcr4 in T cells and regulated by CXCL12 Ab treatment. Notably, CD8+ T cells were significantly increased and activated via Jak/Stat pathway in the AA model but inactivated after CXCL12 Ab treatment. Collectively, these results indicate that humanized CXCL12 Ab is promising for AA treatment via immune modulatory effects.
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| Overall design |
Skin samples were collected from unaffected C3H/HeN mice (negative control, Neg), AA model mice, and AA model mice treated with CXCL12-neutralizing antibody (AA + Ab).
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| Contributor(s) |
Sung J, Noh M, An S, Zheng M, Park I |
| Citation(s) |
39483478 |
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| Submission date |
Jun 09, 2024 |
| Last update date |
Nov 03, 2024 |
| Contact name |
Jong-Hyuk Sung |
| E-mail(s) |
brian99@epibiotech.com
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| Organization name |
Epi Biotech Co., Ltd.
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| Street address |
85 Songdogwahak-ro, Yeonsu-gu
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| City |
Incheon |
| ZIP/Postal code |
21983 |
| Country |
South Korea |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA1121949 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE269455_RAW.tar |
338.8 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
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