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| Status |
Public on Aug 30, 2024 |
| Title |
Gene expression profile at single cell level of iron positive and iron negative cells from subcutaneous implanted syngeneic fibrosarcoma tumors in C57BL/6 mice. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Iron-rich tumor-associated macrophages (iTAMs) are characterized by high intracellular iron, heme metabolism, and expression of the endothelin receptor B. iTAMs promote angiogenesis and immunosuppression in tumors. Heme-mediated degradation of the transcriptional repressor BACH1 induces the iTAM transcriptional program in macrophages.
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| Overall design |
Cells were isolated from subcutaneous implanted syngeneic fibrosarcoma tumors in C57BL/6 mice. Cells were iron enriched using magnetic columns to obtain an rion positive eluate and an iron negative flowthrough. Both fractions were analyzed with scRNAseq.
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| Contributor(s) |
Folkert IW, Arocho WA, Haldar M |
| Citation missing |
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| Submission date |
Jun 05, 2024 |
| Last update date |
Aug 31, 2024 |
| Contact name |
William Molina Arocho |
| E-mail(s) |
wmolin@pennmedicine.upenn.edu
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| Organization name |
University of Pennsylvania
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| Street address |
421 Curie Blvd BRB II/III Rm 436
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| City |
Philadelphia |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA1120499 |
