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| Status |
Public on Oct 09, 2024 |
| Title |
Progesterone increases metabolism via the pentose phosphate pathway in bovine uterine epithelial cells |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
During early pregnancy, glucose is essential for the uterine epithelium and the developing embryo. In cows, studies have shown that progesterone increases the secretion of glucose into the uterine lumen. Nevertheless, how progesterone affects glucose metabolism in the uterine epithelium has been sparsely investigated. Therefore, our objective was to investigate how progesterone influences glucose metabolism in immortalized bovine uterine epithelial (BUTE) cells. Progesterone treatment (10 μM) increased glucose consumption. To gain a more global picture of the effects of progesterone, RNAseq was performed after BUTE cells were treated with vehicle (DMSO) or 10 μM for 24 hours. KEGG analysis indicated that progesterone altered genes associated with metabolic pathways and glutathione metabolism. Manually examining genes unique to specific glucose metabolic pathways identified an increase in the rate-limiting enzyme in the pentose phosphate pathway—glucose-6-phosphate dehydrogenase. Functionally, a major product of the pentose phosphate pathway is NADPH, and progesterone treatment increased NADPH concentrations in BUTE cells. In cows, immunohistochemistry confirmed that glucose-6-phosphate dehydrogenase levels were higher in the uterine epithelium in the luteal phase when progesterone concentrations are high. In conclusion, progesterone increased glucose-6-phosphate dehydrogenase expression and metabolism via the pentose phosphate pathway in the bovine uterine epithelium. This metabolism could provide substrates for cell proliferation, molecules to be secreted into the uterine lumen that supports embryonic development, or maintain reduction/oxidation balance in the uterine epithelium.
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| Overall design |
We previously generated an immortalized bovine uterine epithelial (BUTE) cell line. Here BUTE cells were plated and allowed to grow until 80% confluent. Then the media was removed, rinsed twice with PBS, and the cells were serum and steroid starved in media without phenol red and treated with insulin-like growth factor 1 (IGF1) for 24 hours to stimulate glycogenesis. The media was removed and replaced with serum and steroid-free media containing 50 ng/ml of IGF1, vehicles (DMSO) or 10 μM progesterone for 24 hours. Total RNA was extracted from BUTE cells using the Quick-RNA MiniPrep Plus Kit (Zymo Research) per the manufacturer’s instructions.
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| Contributor(s) |
Berg MD, Braz C, Dean M |
| Citation missing |
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| Submission date |
Jun 04, 2024 |
| Last update date |
Oct 09, 2024 |
| Contact name |
Camila Braz |
| E-mail(s) |
cbraz@illinois.edu
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| Organization name |
University of Illinois Urbana-Champaign
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| Department |
Animal Sciences
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| Street address |
1207 W. Gregory Dr.
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| City |
Urbana |
| State/province |
IL |
| ZIP/Postal code |
61801 |
| Country |
USA |
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| Platforms (1) |
| GPL26012 |
Illumina NovaSeq 6000 (Bos taurus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA1120064 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE269076_Length_normalized_gene_counts.txt.gz |
1.3 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
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