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| Status |
Public on Sep 25, 2024 |
| Title |
Effect of FTO-KD or FTO-OE on gene expression in GH3 cell line |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Growth hormone-secreting pituitary adenomas account for approximately 10% of endocrine pituitary tumors and are pathologically classified into densely granulated adenomas (DGGH) and sparsely granulated adenomas (SGGH). m6A has been reported to play an important role in tumorigenesis and development, but its potential role in the pathogenesis of these two subtypes has not yet been thoroughly investigated. Consequently, we performed MeRIP-seq analysis of pituitary adenomas and the GH3 cell line. We observed that the expression of FTO was significantly increased in SGGH, leading to a reduction in m6A modification and a disruption of desmosome organization.
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| Overall design |
To explore the effect of FTO on gene expression and m6A modification, we established FTO-KD and FTO-OE GH3 cell lines.
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| Contributor(s) |
Jiang X, Zuo Z |
| Citation(s) |
39304899 |
| |
| Submission date |
Jun 04, 2024 |
| Last update date |
Sep 25, 2024 |
| Contact name |
Zhixiang Zuo |
| E-mail(s) |
zuozhx@sysucc.org.cn
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| Organization name |
Sun Yat-sen University
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| Department |
Cancer Center
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| Street address |
No. 651 Dongfeng Dong road
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| City |
Guangzhou |
| ZIP/Postal code |
510000 |
| Country |
China |
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| Platforms (1) |
| GPL25947 |
Illumina NovaSeq 6000 (Rattus norvegicus) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA1120007 |
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