|
| Status |
Public on Oct 02, 2024 |
| Title |
Single Cell Multiomics Profiling for the Mouse Polycystic Kidney Disease |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We performed multiomics analysis; single nucleus RNA-seq (snRNA-seq) combined with ATAC (snATAC-seq) with 10XGenomics Multiome platform to generate cell-type-specific gene expression and chromatin accessibility atlas of the mouse polycystic kidney disease on a time course.
|
| |
|
| Overall design |
We inducedPkd1deletion in the tubular cells of Pkd1fl/fl; Pax8rtTA; TetO-Cremale mice from postnatal day 27 (P27) to 57 (P57) with doxycycline injection. The littermate male controls lacked either thePax8rtTA(P66) ortheTetOCremodule (P100, P130) but were treated with doxycycline in the same manner. The kidneys were harvested and snap-frozen at postnatal day 66 (P66), 100 (P100) or 130 (P130).
|
| |
|
| Contributor(s) |
Muto Y, Humphreys BD |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 28, 2024 |
| Last update date |
Oct 03, 2024 |
| Contact name |
Yoshiharu Muto |
| E-mail(s) |
y.muto@wustl.edu
|
| Organization name |
Washington University in St. Louis
|
| Department |
Medicine / Nephrology
|
| Lab |
Benjamin Humphreys Lab
|
| Street address |
660. S Euclid Ave., CB 8118
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (32)
|
|
| Relations |
| BioProject |
PRJNA1117459 |
Less...
More...