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| Status |
Public on May 27, 2024 |
| Title |
Transcriptomics Provide Insights into Early Responses to Sucrose Signaling in Lupinus albus. |
| Organism |
Lupinus albus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Phosphorus (P) and iron (Fe) deficiency are major limiting factors for plant productivity worldwide. White lupin (Lupinus albus L.) has become a model plant for understanding plant adaptations to P and Fe deficiency, because of its ability to form cluster roots, bottle-brush-like root structures that play an important role in the uptake of P and Fe from soil. However, little is known about the signaling pathways involved in sensing and responding to P and Fe deficiency. Sucrose, sent in increased concentrations from the shoot to the root, has been identified as a long- distance signal of P and Fe deficiencies. To unravel responses to sucrose as a signal, we performed Oxford Nanopore cDNA sequencing of white lupin roots treated with sucrose for 10 min, 15 min, and 20 min, compared to untreated controls. We identified a set of 17 genes, including two bHLH transcription factors, that were upregulated at all three time points of sucrose treatment. GO (gene ontology) analysis revealed enrichment of auxin- and gibberellin-responses as early as 10 min after sucrose addition, and the emerging of ethanol-responses at 20 min of sucrose treatment, indicating a sequential involvement of these hormones in plant responses to sucrose.
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| Overall design |
To unravel responses to sucrose as a signal, we performed Oxford Nanopore cDNA sequencing of white lupin roots treated with sucrose for 10 min, 15 min, and 20 min, compared to untreated controls.
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| Contributor(s) |
Shammi T, Lee YS, Trivedi J, Sierras D, Mansoor A, Maxwell J, Williamson M, Mcmillan M, Chakravarty I, Uhde-Stone C |
| Citation(s) |
39062943 |
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| Submission date |
May 22, 2024 |
| Last update date |
Aug 16, 2024 |
| Contact name |
Claudia Uhde-Stone |
| E-mail(s) |
claudia.stone@csueastbay.edu
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| Organization name |
California State University, East Bay
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| Department |
Biological Sciences
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| Lab |
Stone lab
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| Street address |
25800 Carlos Bee Blvd
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| City |
Hayward |
| State/province |
CA |
| ZIP/Postal code |
94542 |
| Country |
USA |
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| Platforms (1) |
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| Samples (12)
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| GSM8286480 |
L. albus no sucrose (control) replicate 1 |
| GSM8286481 |
L. albus no sucrose (control) replicate 2 |
| GSM8286482 |
L. albus no sucrose (control) replicate 3 |
| GSM8286483 |
L. albus 10 min 10 mM sucrose replicate 1 |
| GSM8286484 |
L. albus 10 min 10 mM sucrose replicate 2 |
| GSM8286485 |
L. albus 10 min 10 mM sucrose replicate 3 |
| GSM8286486 |
L. albus 15 min 10 mM sucrose replicate 1 |
| GSM8286487 |
L. albus 15 min 10 mM sucrose replicate 2 |
| GSM8286488 |
L. albus 15 min 10 mM sucrose replicate 3 |
| GSM8286489 |
L. albus 20 min 10 mM sucrose replicate 1 |
| GSM8286490 |
L. albus 20 min 10 mM sucrose replicate 2 |
| GSM8286491 |
L. albus 20 min 10 mM sucrose replicate 3 |
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| Relations |
| BioProject |
PRJNA1114769 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE268152_CPM_genes_Lupinus_albus_sucrose.csv.gz |
970.8 Kb |
(ftp)(http) |
CSV |
| GSE268152_DESeq2_all_genes_t10-t15-t20_annotated.csv.gz |
2.5 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
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