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| Status |
Public on Jun 13, 2011 |
| Title |
qRT-PCR of ex vivo Salmonella enterica in TLR4 minus/plus macrophage background |
| Organism |
Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S |
| Experiment type |
Expression profiling by RT-PCR
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| Summary |
A major virulence factor in S. enterica is the pathogenicity island Spi2. The goal of the experiment was to determine the impact of the toll-like receptor 4 (TLR-4) on expression of Spi2 in S. enterica wild type (WT) and triple mutant strain (TMS) with deletions in SPI-1 TTSS, SPI-2 TTSS, flagella. The stain 14028 was used for the experiment. Cells were grown in a TLR-4 wildtype (plus) or deleted (minus) macrophage background. Total RNA was extracted and processed by qRT-PCR.
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| Overall design |
Two strains (WT and TMS) grown under 2 conditions (TLR-4 wildtype (plus) or TLR-4 deleted (minus)) - total 4 samples were analyzed in the study.
All PCRs were done in quadruplicates, located on the same PCR plate. For each quadruplicates the median value was calculated and used as a data point for further normalization. A set of controls was selected for all data sets using the following criteria: the data points for the controls should have Cp <40 and be present in all the samples.
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| Contributor(s) |
Heffron F, Jones M, Dracheva T |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jan 21, 2011 |
| Last update date |
Mar 22, 2012 |
| Contact name |
John Braisted |
| E-mail(s) |
jbraisted@jcvi.org
|
| Organization name |
J Craig Venter Institute
|
| Department |
PFGRC
|
| Lab |
PFGRC_EXTSW
|
| Street address |
9704 Medical Center Dr
|
| City |
Rockville |
| State/province |
MD |
| ZIP/Postal code |
20850 |
| Country |
USA |
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| Platforms (1) |
| GPL11111 |
Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S 0.3K |
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| Samples (4)
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| GSM659306 |
qRT-PCR of ex vivo Salmonella enterica wildtype strain 14028 grown in TLR-4 (plus) macrophage background |
| GSM659307 |
qRT-PCR of ex vivo Salmonella enterica wildtype strain 14028 grown in TLR-4 (minus) macrophage background |
| GSM659368 |
qRT-PCR of ex vivo Salmonella enterica triple mutant strain 14028 grown in TLR-4 (plus) macrophage background |
| GSM659369 |
qRT-PCR of ex vivo Salmonella enterica triple mutant strain 14028 grown in TLR-4 (minus) macrophage background |
|
| Relations |
| BioProject |
PRJNA136063 |
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