Expression profiling by high throughput sequencing
Summary
As no one previously examined urine-derived cells from bladder cancer patients, we performed scRNAseq to profile the diversity of these cells and their transcriptional profiles. We used scRNAseq to compare the profiles of urine-derived cells to matched tumor cells and PBMC from bladder cancer patients.
Overall design
We spun down urine-derived cells from urine, isolated PBMC using Ficoll-based separation, and dissociated cells from tumor. We analyzed each specimen individually using scRNAseq.
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