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| Status |
Public on Sep 13, 2024 |
| Title |
Bulk RNA-seq analysis of HepG2 exposed to oleic and palmitic acid |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We hypothesized that exposure to fatty acids in metabolic dysfunction-associated steatohepatitis-like environment will profoundly affect gene expression of hepatocytes. More precisely, we wish to investigate expression of genes related to ferroptosis, i.e. an iron-catalyzed form of cell death through lethal lipid peroxidation.
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| Overall design |
We aimed to study the effect of fatty acid supplementation in a metabolic dysfunction-associated steatohepatitis-like environment on gene expression of HepG2 cells profiled with bulk mRNA-sequencing. HepG2 cells were exposed for 48 hours to oleic acid (100microM) and palmitic acid (50microM), as well as hyperglycemia (4.5 mg/mL), hyperinsulinemia (100 nM), tumor necrosis factor alpha (50 ng/mL), interleukin 1-beta (25 ng/mL) and transforming growth factor-beta (8ng/mL). Control samples were exposed to solvents needed to dissolve oleic acid and palmitic acid in medium.
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| Citation(s) |
39060422 |
| BioProject |
PRJNA1110746 |
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| Submission date |
May 16, 2024 |
| Last update date |
Sep 14, 2024 |
| Contact name |
Cédric Peleman |
| E-mail(s) |
cedric.peleman@uantwerpen.be
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| Organization name |
University of Antwerp
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| Street address |
Universiteitsplein 1
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| City |
Antwerpen |
| ZIP/Postal code |
2610 |
| Country |
Belgium |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (12)
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