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Series GSE267694 Query DataSets for GSE267694
Status Public on Sep 13, 2024
Title Bulk RNA-seq analysis of HepG2 exposed to oleic and palmitic acid
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We hypothesized that exposure to fatty acids in metabolic dysfunction-associated steatohepatitis-like environment will profoundly affect gene expression of hepatocytes. More precisely, we wish to investigate expression of genes related to ferroptosis, i.e. an iron-catalyzed form of cell death through lethal lipid peroxidation.
 
Overall design We aimed to study the effect of fatty acid supplementation in a metabolic dysfunction-associated steatohepatitis-like environment on gene expression of HepG2 cells profiled with bulk mRNA-sequencing. HepG2 cells were exposed for 48 hours to oleic acid (100microM) and palmitic acid (50microM), as well as hyperglycemia (4.5 mg/mL), hyperinsulinemia (100 nM), tumor necrosis factor alpha (50 ng/mL), interleukin 1-beta (25 ng/mL) and transforming growth factor-beta (8ng/mL). Control samples were exposed to solvents needed to dissolve oleic acid and palmitic acid in medium.
 
Citation(s) 39060422
BioProject PRJNA1110746
Submission date May 16, 2024
Last update date Sep 14, 2024
Contact name Cédric Peleman
E-mail(s) cedric.peleman@uantwerpen.be
Organization name University of Antwerp
Street address Universiteitsplein 1
City Antwerpen
ZIP/Postal code 2610
Country Belgium
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (12)
GSM8273248 Control B4
GSM8273249 Control B5
GSM8273250 OA_PA B7

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE267694_RAW.tar 3.5 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA

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