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| Status |
Public on Aug 19, 2024 |
| Title |
Differential compatibilities between rhizobium strains and cultivated and wild soybeans revealed by anatomical and RNA-seq analyses. |
| Organisms |
Glycine max; Glycine soja |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Various species of rhizobium establish compatible symbiotic relationships with soybean (Glycine max) leading to the formation of nitrogen-fixing nodules in roots. The formation of functional nodules is mediated through complex developmental and transcriptional reprogramming that involves the activity of thousands of plant genes. However, host transcriptome responses that determine the outcome of the symbiotic interactions leading to the formation of functional or non-functional nodules remain unexplored. In this study, we investigated differential compatibilities between rhizobium strains (Bradyrhizobium diazoefficiens USDA 110Bradyrhizobium sp. strain LVM 105) and cultivated and wild soybeans. The nodulation assays revealed that both USDA 110 and LVM 105 strains effectively nodulate G. soja but only USDA 110 can form symbiotic relationships with Williams 82. RNA-seq data revealed that USDA 110 and LVM 105 induce distinct transcriptome programming in functional mature nodules formed on G. soja roots where genes involved in nucleosome assembly, DNA replication, regulation of cell cycle, and defense responses play key roles. Transcriptome comparison also suggested that activation of genes associated with cell wall biogenesis and organization and defense responses together with downregulation of genes involved in the biosynthesis of isoprenoids and antioxidant stress are associated with the formation of non-functional nodules on Williams 82 roots. Moreover, our analysis implies that increased activity of genes involved in oxygen binding, amino acid transport, and nitrate transport differentiates between fully-developed nodules in cultivated versus wild soybeans.
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| Overall design |
In this study, we investigated transcriptome reprogramming in 25-day-old nodules induced by USDA 110 and LVM 105 in William 82 and G. soja(3 replicates per treatment) using RNA-seq and determined the biological processes and molecular functions controlling soybean response to nodulation by USDA 110 and LVM 105.
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| Contributor(s) |
Hewezi T, Krishnan H, Bahrami Zadegan S |
| Citation(s) |
39290740 |
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| Submission date |
May 16, 2024 |
| Last update date |
Oct 09, 2024 |
| Contact name |
Tarek Hewezi |
| Organization name |
University of Tennessee, Knoxvile
|
| Street address |
2621 Morgan Circle
|
| City |
Knoxville |
| ZIP/Postal code |
37996 |
| Country |
USA |
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| Platforms (2) |
| GPL28801 |
Illumina NovaSeq 6000 (Glycine max) |
| GPL34481 |
Illumina NovaSeq 6000 (Glycine soja) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA1112310 |
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