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| Status |
Public on Jul 03, 2024 |
| Title |
The role of the gene Mest during mouse digit tip regeneration |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The distal mouse digit tip undergoes complex tissue regeneration following amputation, a process facilitated by the formation of a cellular structure called the blastema. Through single-cell RNA sequencing of the blastema, we identified the gene Mest (mesoderm specific transcript) highly expressed in a subset of blastemal fibroblasts. To determine if Mest expression is necessary for mouse digit tip regeneration, we analyzed Mest wildtype (WT) versus homozygous knockout (KO) post amputation digits. Through single-cell sequencing and FACS analysis of WT and KO regenerating tissues, we determined that this phenomenon was due to a prolonged immune response in the KO digits.
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| Overall design |
Cohorts of adult Mest genetic knock-out (KO) mice and Mest wild-type (WT) mice underwent digit tip amputation; 12 days post-amputation, digit tip blastemas were collected and used for single cell RNA sequencing and analysis.
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| Contributor(s) |
Lehoczky JA |
| Citation(s) |
38915675 |
| |
| Submission date |
May 15, 2024 |
| Last update date |
Jul 04, 2024 |
| Contact name |
Jessica A Lehoczky |
| E-mail(s) |
jlehoczky@bwh.harvard.edu
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| Organization name |
Brigham and Women's Hospital
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| Department |
Orthopedic Surgery
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| Street address |
60 Fenwood Rd.
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA1111844 |
