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| Status |
Public on May 31, 2024 |
| Title |
N-acetylglucosamine (GlcNAc) sensor Ngs1 is essential for GlcNAc-involved biological control potential of fungal insecticide Beauveria bassiana |
| Organism |
Beauveria bassiana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Beauveria bassiana is a popular and eco-friendly biopesticide. Sensing N-acetylglucosamine (GlcNAc) , a building block of both insect exoskeleton and fungal cell wall, might play a vital role during B. bassiana pathogen-pest interaction. Previous studies in Candida albicans have demonstrated that a GlcNAc sensor (termed as Ngs1) was in response to GlcNAc signal sensing and transportation. Herein, we found a Ngs1 homology in B. bassiana, and reported the alteration of transcriptomic profiles between wild-type strain and Ngs1-deletion mutants grown on cuticle broth (CB) supplemented with 1% cuticle powder of Locusta migratoria exoskeleton, followed by shaking at 150 rpm for 5 days at 25 ℃.
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| Overall design |
First, Ngs1-deletion mutants were constructed through homologous-recombination strategy; then, aliquots of 50 mL 10^6 conidia/mL cuticle broth (CB) containing 1% cuticle powder were incubated by shaking at 150 rpm for 5 days at 25 ℃. The cultures were centrifuged by 13,200×g for 1 min at 4 ℃. Hyphal cultures were collected and used for total RNA extracted; subsequently, the extracted RNA were sent to Novogene Company (Beijing, China) for RNA sequencing using Illumina Hiseq 2000.
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| Contributor(s) |
Zhang L, Yang W |
| Citation missing |
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| Submission date |
May 13, 2024 |
| Last update date |
May 31, 2024 |
| Contact name |
Long-Bin zhang |
| E-mail(s) |
11207029@zju.edu.cn
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| Organization name |
Fuzhou University
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| Department |
College of Biological Science and Engineering
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| Lab |
The Key Laboratory of Marine Enzyme Engineering of Fujian Province
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| Street address |
Jianping Road
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| City |
Fuzhou |
| State/province |
Fujian |
| ZIP/Postal code |
360108 |
| Country |
China |
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| Platforms (1) |
| GPL16038 |
Illumina HiSeq 2000 (Beauveria bassiana) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA1111093 |
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