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Series GSE267323 Query DataSets for GSE267323
Status Public on May 18, 2024
Title High-Resolution Spatial Transcriptomics Reveals Stroma Damage in Human Ovarian Tissue Response to Cryopreservation
Organism Homo sapiens
Experiment type Other
Summary Cryopreservation is an important technique for preserving fertility potential through ovarian tissue banking. Slow freezing has traditionally been the standard protocol used for ovarian tissue cryopreservation. However, vitrification, as an emerging method, is considered to be an alternative to slow freezing, thus generating some debate in the field. This study utilizes high-resolution spatial transcriptomics to comprehensively profile and compare the molecular impacts of two cryopreservation methods - slow freezing and vitrification - on human ovarian tissues at an unprecedented resolution. Over 135,000 spots were profiled from Fresh and cryopreserved ovarian tissue slices, identifying 8 major cell types and 25 subtypes through marker expression. Detailed spatial localization patterns revealed heterogeneous stromal cell subpopulations and ovarian structures. Transcriptional profiling showed that both cryopreservation methods significantly repressed gene expression, indicative of tissue damage. However, slow freezing induced a broader pro-survival inflammatory and tissue remodeling response compared to vitrification. Gene set enrichment analysis identified elevated ribosomal processes, inflammatory signaling pathways like TNF-?/NF-?B and IL-6/STAT3, as well as extracellular matrix (ECM) organization and collagen synthesis as dominant response themes in slow frozen tissue, which were observed to a lesser degree in vitrified tissue. Key response drivers included MYC, TIMP1, and DCN, with stromal cells located in the cortical regions showing the strongest pathway enrichment. Ribosomal gene expression displayed spatial gradients and supported stromal responses after freezing. We finally demonstrated signaling pathway differences between the two cryopreservation methods, noting TGF-? as a highly possible pathway responsible for coordinating with certain endothelial subgroups to enhance ECM deposition. In summary, this study provides a comprehensive atlas of cell types and their spatial structures in human ovary. It reveals that slow freezing elicits a strong but balanced inflammatory and tissue regenerative state compared to the vitrification. It also offers insights on cryoinjury to ovarian stroma, which may guide optimization of ovarian tissue cryopreservation protocols for clinical applications.
Overall design Human ovarian tissues were donated from one patient aged 35 years who underwent oophorectomy due to cervical cancer. We conducted Stereo-seq profilling of transcriptomic data on three tissue pieces stored under different conditions. According to the Stereo-Seq protocol, one piece of fresh group tissues was fixed in O.C.T. compound (Tissue Tek) for subsequent sequencing, tissues of the two cryopreservation groups were processed after freezing and thawing.
Contributor(s) Zhao P, Ma K, Li Y, Guo C, Du H, Wu H, Li X, Liu K, Wang Y, Liu K
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date May 13, 2024
Last update date May 18, 2024
Contact name Kun MA
Phone 56493222
Organization name The University of Hong Kong
Street address No 12 Kam Ling Court Whitty Street Sai Wan HK Island
City HK
State/province Hong Kong
ZIP/Postal code 000000
Country Hong Kong
Platforms (1)
GPL29480 DNBSEQ-T7 (Homo sapiens)
Samples (3)
GSM8263906 Ovary cortex-medulla, fresh, slice 1 & 2
GSM8263907 Ovary cortex-medulla, slow-frozen, slice 1 & 2
GSM8263908 Ovary cortex-medulla, vitrified, slice 1 & 2
BioProject PRJNA1111069

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Supplementary file Size Download File type/resource
GSE267323_RAW.tar 16.9 Gb (http)(custom) TAR (of H5, TAR, TXT)
GSE267323_bin50.rds.gz 212.2 Mb (ftp)(http) RDS
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