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| Status |
Public on May 21, 2024 |
| Title |
Upregulation of inflammatory genes and pathways links obesity to severe COVID-19 |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Obesity is a risk factor for developing severe COVID-19. However, the mechanism underlying obesity-accelerated COVID-19 remains unclear. Here, we report results from a study in which K18-hACE2 mice were fed an obesity-inducing western diet (WD) for over 3 months before intranasal infection with SARS-CoV2. After infection, the WD-fed K18-hACE2 mice lost more body weight and had more severe lung inflammation than normal chow (NC)-fed mice. Bulk RNAseq analysis of lungs and adipose tissue revealed that a diverse landscape of various immune cells, inflammatory markers, and pathways are upregulated in obese COVID-19 patients or the WD-fed K18-hACE2 mice when compared with their respective control groups. When compared with infected NC-fed mice in the lung, the infected WD-fed mice had upregulation of IL-6, a well-established marker for severe COVID-19. These results indicate that obesity-accelerated severe COVID-19 caused by SARS-CoV-2 infection in the K18-hACE2 mouse model can be used for dissecting the cellular and molecular mechanisms underlying pathogenesis. Furthermore, in the transcriptome analysis of the lung and adipose tissue obtained from deceased COVID-19 patients, we found upregulation of an array of genes and pathways associated with Inflammation. Both the K18-hACE2 mouse model and human COVID-19 patient data support a link between inflammation and an obesity-accelerated COVID-19 disease phenotype.
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| Overall design |
Comparitive gene expression analysis was done using bulk RNA-seq data comparing NC (normal chow) and WD (western diet to induce obesity) fed K18-hACE2 mice (adipose tissue and lung tissue) infected with SARS-CoV-2 (WA).
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| Contributor(s) |
Currey J, Qin X, Kolls JK, Shamima Khatun M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
May 08, 2024 |
| Last update date |
May 21, 2024 |
| Contact name |
Jay Kolls |
| Organization name |
Tulane University
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| Department |
Medicine
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| Lab |
Center for Translational Research in Infection and Inflammation
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| Street address |
1430 Tulane Ave
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| City |
New Orleans |
| State/province |
LA |
| ZIP/Postal code |
70112 |
| Country |
USA |
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| Platforms (2) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA1109355 |
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