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| Status |
Public on Jan 19, 2011 |
| Title |
Expression data from post mortem porcine skeletal muscle |
| Organism |
Sus scrofa |
| Experiment type |
Expression profiling by array
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| Summary |
We set up a pilot study using Affymetrix Gene Chip® Porcine Genome Arrays to evaluate the impact of time lags from death on gene expression profiling of porcine skeletal muscle at four post mortem time points (up to 24 hrs) during the routine processing of fresh tights
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| Overall design |
Post mortem skeletal muscle samples were obtained from three commercial hybrid pigs of female sex (of about 160 kg each) raised in the same commercial farm and slaughtered under normal conditions at the same abattoir within 1 minute of each other after stunning by CO2 (concentration 87%) using a dip lift system (Butina, Denmark). A portion of semimembranosus muscle (3-5 g) was sampled from the left legs at 20 minutes (T0) after death following the normal operation of the abattoir. Other samples were collected from the same muscle at the same position after 2 (T1), 6 (T2) and 24 (T3) hrs post mortem in the same abattoir following the cold chain at the abattoir until 4 °C. After sampling, tissues were snap frozen in liquid nitrogen and stored at -80 °C till RNA extraction.
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| Contributor(s) |
Fontanesi L, Calò DG |
| Citation(s) |
21414728 |
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| Submission date |
Jan 19, 2011 |
| Last update date |
May 03, 2013 |
| Contact name |
Giuliano Galimberti |
| E-mail(s) |
giuliano.galimberti@unibo.it
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| Organization name |
Università di Bologna
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| Street address |
Via delle Belle Arti, 41
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| City |
Bologna |
| ZIP/Postal code |
40126 |
| Country |
Italy |
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| Platforms (1) |
| GPL3533 |
[Porcine] Affymetrix Porcine Genome Array |
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| Samples (12)
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| GSM657204 |
skeletal muscle sample at T0, pig 1 |
| GSM657205 |
skeletal muscle sample at T0, pig 2 |
| GSM657206 |
skeletal muscle sample at T0, pig 3 |
| GSM657207 |
skeletal muscle sample at T1, pig 1 |
| GSM657208 |
skeletal muscle sample at T1, pig 2 |
| GSM657209 |
skeletal muscle sample at T1, pig 3 |
| GSM657210 |
skeletal muscle sample at T2, pig 1 |
| GSM657211 |
skeletal muscle sample at T2, pig 2 |
| GSM657212 |
skeletal muscle sample at T2, pig 3 |
| GSM657213 |
skeletal muscle sample at T3, pig 1 |
| GSM657214 |
skeletal muscle sample at T3, pig 2 |
| GSM657215 |
skeletal muscle sample at T3, pig 3 |
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| Relations |
| BioProject |
PRJNA136155 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE26701_RAW.tar |
22.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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