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| Status |
Public on Oct 01, 2024 |
| Title |
MEOX2 Regulates Adipogenic Differentiation of Adipose-Derived Stromal Vascular Fractions through PI3K/AKT Signaling Pathway |
| Organism |
Ovis aries |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The fat tail of sheep is an important organ that has evolved to adapt to extreme environmental conditions. Mesenchyme homeobox 2 (MEOX2) can inhibit adipogenic differentiation of stromal vascular fractions (SVFs) isolated from ovine tail adipose tissue. However, the underlying mechanism through which MEOX2 regulates ovine adipogenesis remains unclear. Therefore, this study aims to employ RNA sequencing (RNA-seq) to explore the downstream genes regulated by MEOX2 during the adipogenic differentiation of ovine SVFs. Based on RNA sequencing, several differentially expressed genes (DEGs) were identified in response to MEOX2 overexpression. The PI3K/AKT signaling pathway was significantly enriched in DEGs, indicating its importance in mediating fat accumulation regulated by MEOX2. Additionally, the PI3K/AKT signaling pathway activation was found to be crucial for ovine SVF adipogenic differentiation. Mechanistically, MEOX2 inhibited the PI3K/AKT pathway. These findings highlight the significance of the PI3K/AKT signaling pathway during ovine SVF adipogenic differentiation. Furthermore, they shed light on the involvement of MEOX2 in this differentiation via the PI3K/AKT pathway. Our findings provide novel insights into the critical role of MEOX2 as an adipogenetic differentiation regulator, potentially enhancing our understanding of ovine fat development and its regulatory processes.
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| Overall design |
After overexpression of MEOX2 in sheep SVFs, transcriptome sequencing was performed to screen the differential genes compared with the control group.
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| Citation missing |
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| Submission date |
May 06, 2024 |
| Last update date |
Oct 01, 2024 |
| Contact name |
弼时 赵 |
| E-mail(s) |
zhaobishi2021@gmail.com
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| Phone |
18035434746
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| Organization name |
Shanxi Agricultural University
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| Street address |
Mingxian South Road
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| City |
Taigu |
| State/province |
--- Select One --- |
| ZIP/Postal code |
030801 |
| Country |
China |
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| Platforms (1) |
| GPL27721 |
Illumina NovaSeq 6000 (Ovis aries) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA1108433 |
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