|
| Status |
Public on Feb 04, 2011 |
| Title |
Efficient Generation of Transgene-Free Induced Pluripotent Stem Cells from Normal and Neoplastic Bone Marrow and Cord Blood Mononuclear Cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
global gene expression were compared among human blood iPSC, human fibroblas iPSC, human embryonic stem cells, human bone marrow MNC and human forskin fibroblast
Reprogramming blood cells to induced pluripotent stem cells (iPSCs) provides a novel tool for modeling blood diseases in vitro. we demonstrated that iPSCs free of transgene and vector sequences could be efficiently generated from human bone marrow and cord blood mononuclear cells using non-integrating episomal vectors. The reprogramming described here is up to 100 times more efficient, occurs 1 to 3 weeks faster as compared to the reprogramming of fibroblasts, and does not require isolation of progenitors or multiple rounds of transfection. This approach provides an opportunity to explore banked normal and diseased cord blood and bone marrow samples without the limitations associated with virus-based methods.
|
| |
|
| Overall design |
Compare the global gene expression of iPSs from different sources, ESCs and Somatic cells
|
| |
|
| Contributor(s) |
Hu K, Yu J, Suknuntha K, Tian S, Montgomery K, Choi K, Stewart R, Thomson JA, Slukvin II |
| Citation(s) |
21296996 |
| |
| Submission date |
Jan 18, 2011 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Igor Slukvin |
| E-mail(s) |
islukvin@wisc.edu
|
| Phone |
608-263-0058
|
| Organization name |
University of Wisconsin
|
| Department |
Department of Pathology and Laboratory Medicine
|
| Street address |
1220 Capitol Court
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (19)
|
|
| Relations |
| BioProject |
PRJNA136421 |
Less...
More...