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| Status |
Public on May 14, 2024 |
| Title |
Male autism spectrum disorder is linked to brain aromatase disruption by prenatal BPA in multimodal investigations and 10HDA ameliorates the related mouse phenotype (10HDA) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Primary cortical neurons were obtained from male Cyp19-EGFP mouse embryos at gestational day 15.5. Embryos were genotyped for SRY to determine sex, and only male embryos were used. Cells were seeded in 24-well plates containing 12 mm glass coverslips, coated with 100 µg/mL poly D-lysine to a density of ~0.45 x 106 cells/well and incubated in a humidified CO2 incubator (5% CO2, 37°C). Primary cortical from Cyp19-EGFP mouse embryos were obtained on GD 15.5. Neuronal cell cultures were treated with vehicle or 1mM 10HDA (Matreya, PA ,USA)
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| Overall design |
To investigate the effect of 10HDA on RNA expression in cortical primarly cell culture (obtained from Cyp19-EGFP mice at gestational day 15.5), we treated primary cortical neurons obtained from mice with either vehicle (DSMO) or 1mM 10HDA.
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| Web link |
https://www.nature.com/articles/s41467-024-48897-8
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| Contributor(s) |
Boon W |
| Citation(s) |
39112449 |
| |
| Submission date |
May 01, 2024 |
| Last update date |
Aug 24, 2024 |
| Contact name |
Wah Chin Boon |
| E-mail(s) |
wah.chin.boon@florey.edu.au
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| Phone |
0407640787
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| Organization name |
The Florey Institute of Neuroscience and Mental Health
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| Street address |
30 Royal Parade Parkville
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| City |
Melbourne |
| State/province |
Victoria |
| ZIP/Postal code |
3052 |
| Country |
Australia |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA1106911 |
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