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Series GSE266250 Query DataSets for GSE266250
Status Public on May 10, 2024
Title Pericytes and perivascular fibroblasts contribute to central nervous system fibrosis in a region dependent manner(DH SmartSeq)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Fibrotic scar tissue formation is conserved throughout the central nervous system in humans and mice, and impairs tissue regeneration and functional recovery. However, the origin of scar-forming stromal fibroblasts is controversial. Here, we show that stromal fibroblasts found after spinal cord injury derive from two populations of perivascular cells that are anatomically and transcriptionally defined as pericytes and perivascular fibroblasts. We identify two distinct perivascular cell populations, which activate and transcriptionally converge on the generation of stromal myofibroblasts after injury. Our results suggest potential targets to improve tissue regeneration and functional recovery after central nervous system injuries.
 
Overall design We crossed GLAST-CreERT2;R26R-tdTomato mice to the Pdgfrb-eGFP reporter line, which drives EGFP expression in all PDGFRb+ perivascular cells (pericytes, vSMCs and fibroblasts). We isolated GLAST-expressing perivascular cells from the uninjured spinal cord and 5 days after spinal cord injury by fluorescence activated cell sorting (FACS) based on tdTomato and EGFP expression. To define the molecular identity and compare GLAST-expressing perivascular cells (tdTomato+EGFP+) to other perivascular cells, we also sorted dTomato-EGFP+ cells. Sorted cells were subjected single cell sequencing using the SmartSeq 3 protocol.
 
Contributor(s) Daniel H, Anaïs J, Christian G
Citation(s) 38849523
Submission date Apr 30, 2024
Last update date Aug 08, 2024
Contact name Christian Göritz
E-mail(s) christian.goeritz@ki.se
Organization name Karolinska Institutet
Department CMB
Street address Solnavägen 9
City Solna
ZIP/Postal code 17165
Country Sweden
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (503)
GSM8243332 Spinal cord, Uninjured, 209s_F01
GSM8243333 Spinal cord, Uninjured, 209s_F03
GSM8243334 Spinal cord, Uninjured, 209s_F08
Relations
BioProject PRJNA1106449

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Supplementary file Size Download File type/resource
GSE266250_NN_Glast_B_d0d3d5_count_matrix.csv.gz 15.6 Mb (ftp)(http) CSV
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Raw data are available in SRA
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