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| Status |
Public on Apr 29, 2024 |
| Title |
Influence of DNA-methylation at multiple stages of limb chondrogenesis |
| Organism |
Gallus gallus |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
Limb development is a well-established model for understanding cell fate decisions, and the formation of skeletal elements is coordinated through a sequence of events that control chondrogenesis spatiotemporally. It has been established that epigenetic control participates in cartilage maturation. Our research has shown for the first time that the inhibition of DNA methylation in interdigital tissue with 5-azacytidine results in the formation of an ectopic digit. This discovery suggested that DNA methylation dynamics could regulate the fate of cells between chondrogenesis and cell death during autopod development. We sequenced WGBS libraries from in-vivo interdigital tissue treated with 5-azacytidine or DMSO to identify genome-wide methylation changes during the early stages of 5-azacytidine-induced chondrogenesis.
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| Overall design |
Whole Genome Bisulfite Sequencing libraries (WGBS) from interdigital tissue implanted beads, soaked in 250µM 5-azacytidine or DMSO for 4 hours.
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| |
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| Contributor(s) |
Pérez-Maldonado MA, González-González XA, Chimal-Monroy J, Marín-Llera JC |
| Citation missing |
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| Submission date |
Apr 29, 2024 |
| Last update date |
Apr 30, 2024 |
| Contact name |
Mario Alberto Perez-Maldonado |
| E-mail(s) |
marioperez@uadec.edu.mx
|
| Phone |
528661372195
|
| Organization name |
UNAM
|
| Department |
Institute of Biomedical Research
|
| Lab |
Chimal-Monroy Lab
|
| Street address |
Circuito Mario de la Cueva s/n
|
| City |
Mexico City |
| State/province |
Mexico City |
| ZIP/Postal code |
04510 |
| Country |
Mexico |
| |
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| Platforms (1) |
| GPL26853 |
Illumina NovaSeq 6000 (Gallus gallus) |
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| Samples (4)
|
| GSM8240949 |
interdigital tissue implanted beads, DMSO_rep1 |
| GSM8240950 |
interdigital tissue implanted beads, 5-Azacitidine_rep1 |
| GSM8240951 |
interdigital tissue implanted beads, DMSO_rep2 |
| GSM8240952 |
interdigital tissue implanted beads, 5-Azacitidine_rep2 |
|
| Relations |
| BioProject |
PRJNA1105916 |
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