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| Status |
Public on Jan 06, 2011 |
| Title |
Killer cell Ig-like receptor (KIR) 3DL1 downregulation enhances inhibition of type 1 diabetes by autoantigen-specific regulatory T cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Varied genes may be responsible for the functional differences of distinct subsets of T cells. As a result, it is possible that regulatory T cells and pathogenic T cells may display a different set of gene profile regulating their functional status.
To identify genes contributing to the functional role of T cells, we used microarrays to examine the global gene expression in regulatory T cells, pathogenic T cells, and control non-regulatory, non-pathogenic T cells.
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| Overall design |
BDC, N79, N206 and NR206 CD4+ T cells were cultured in RPMI medium plus 5% fetal bovine serum in vitro. N206 and NR206 were used as Tregs, BDC were used as Tpath cells and N79 were used as neutral control cells . BDC, N79, N206 and NR206 were cultured for RNA extraction and hybridization on Affymetrix microarrays.
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| Contributor(s) |
Qin H, Wang Z, Du W, Lee W, Wu X, Riggs AD, Liu C |
| Citation(s) |
21245333 |
| |
| Submission date |
Jan 05, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Xiwei Wu |
| E-mail(s) |
xwu@coh.org
|
| Organization name |
City of Hope National Medical Center
|
| Department |
Computational and Quantitative Medicine
|
| Street address |
1500 E. Duarte Rd.
|
| City |
Duarte |
| State/province |
CA |
| ZIP/Postal code |
91010 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA136561 |
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