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| Status |
Public on May 15, 2024 |
| Title |
Gene expression alterations in primary choroid plexus epithelial cells infected with JCPyV |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The human polyomavirus, JCPyV, establishes a lifelong persistent infection in the peripheral organs of a majority of the human population worldwide. Patients who are immunocompromised due to underlying infections, cancer, or to immunomodulatory treatments for autoimmune disease are at risk for developing progressive multifocal leukoencephalopathy (PML) when the virus invades the CNS and infects macroglial cells in the brain parenchyma. It is not yet known how the virus enters the CNS to cause disease. The blood-choroid plexus barrier is a potential site of virus invasion as the cells that make up this barrier are known to be infected with virus both in vivo and in vitro. To understand the effects of virus infection on these cells we challenged primary choroid plexus epithelial cells with JCPyV and profiled changes in host gene expression. We found that JC infection induced the expression of proinflammatory chemokines and downregulated junctional proteins essential for maintaining blood-CSF and blood-brain barrier function. These data contribute to our understanding of how virus infection of the choroid plexus can modulate the host cell response to neuroinvasive pathogens.
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| Overall design |
Gene expression profiling analysis of primary human choroid plexus epithelial cells comparing in triplicate cells infected with human polyomavirus JCPyV with control uninfected cells, 7 days post infection. 3 replicates per condition, 6 overall total samples.
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| Contributor(s) |
Haley SA, Atwood WJ, Schorl C |
| Citation(s) |
38874395 |
| |
| Submission date |
Apr 17, 2024 |
| Last update date |
Aug 19, 2024 |
| Contact name |
Sheila Haley |
| E-mail(s) |
sheila_haley@brown.edu
|
| Organization name |
Brown University
|
| Department |
MCB
|
| Street address |
70 Ship Street
|
| City |
Providence |
| State/province |
RI |
| ZIP/Postal code |
02903 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL23126 |
[Clariom_D_Human] Affymetrix Human Clariom D Assay [transcript (gene) version] |
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| Samples (6)
|
| GSM8214825 |
CPEP Uninfected, biological replicate 1 |
| GSM8214826 |
CPEP Uninfected, biological replicate 2 |
| GSM8214827 |
CPEP Uninfected, biological replicate 3 |
| GSM8214828 |
CPEP JCPyV infected, biological replicate 1 |
| GSM8214829 |
CPEP JCPyV infected, biological replicate 2 |
| GSM8214830 |
CPEP JCPyV infected, biological replicate 3 |
|
| Relations |
| BioProject |
PRJNA1101535 |
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