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Series GSE264098 Query DataSets for GSE264098
Status Public on Apr 16, 2024
Title CEBPA restricts alveolar type 2 cell plasticity during development and injury-repair [scATAC-seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Cell plasticity theoretically extends to all possible cell types, but naturally decreases as cells differentiate, whereas injury-repair re-engages the developmental plasticity. Here we show that the lung alveolar type 2 (AT2)-specific transcription factor (TF), CEBPA, restricts AT2 cell plasticity in the mouse lung. AT2 cells undergo transcriptional and epigenetic maturation postnatally. Without CEBPA, both neonatal and mature AT2 cells reduce the AT2 program, but only the former reactivate the SOX9 progenitor program. Sendai virus infection bestows mature AT2 cells with neonatal plasticity where Cebpa mutant, but not wild type, AT2 cells express SOX9, as well as more readily proliferate and form KRT8/CLDN4+ transitional cells. CEBPA promotes the AT2 program by recruiting the lung lineage TF NKX2-1. The temporal change in CEBPA-dependent plasticity reflects AT2 cell developmental history. The ontogeny of AT2 cell plasticity and its transcriptional and epigenetic mechanisms have implications in lung regeneration and cancer.
Overall design FACS-purified lung epithelial, immune, endothelial, and mesenchymal cells were processed using the 3′ Library and Gel Bead Kit following the manufacturer’s users guide (v2 rev D) on the Chromium Single-Cell Gene Expression Solution Platform (10X Genomics).
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Contributor(s) Hassan D, Chen J
Citation(s) 38755149
Submission date Apr 16, 2024
Last update date May 21, 2024
Contact name Dalia Hassan
Phone 3467146254
Organization name MD Anderson Cancer Center
Department Pulmonary Medicine
Lab Jichao Chen
Street address 6565 MD Anderson Blvd, Z9.4040, Z9.4040
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (4)
GSM8210407 E16.5_WT_lung
GSM8210408 E18.5_WT_lung
GSM8210409 P8_WT_lung
BioProject PRJNA1100986

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Supplementary file Size Download File type/resource
GSE264098_RAW.tar 4.9 Gb (http)(custom) TAR (of BROADPEAK, BW, RDS)
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Raw data are available in SRA

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