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| Status |
Public on Oct 15, 2024 |
| Title |
Chemoradiation responses in esophageal cancer are mediated by temporal dynamics in the tumor ecosystem |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Understanding the temporal dynamics of the esophageal adenocarcinoma (EAC) ecosystem during chemoradiotherapy (CRT) is key for improving the low clinical response rates. Here, we report single cell RNA-seq data of 302,035 cells from 83 longitudinal samples collected from 47 patients to study both pre-existing programs and reprogramed cells that are associated with CRT responses. CRT good-responders showed a lymphoid-activated ecotype with higher tumor-reactive lymphoid cells and activation of interferon pathways. The cancer cells from good-responders showed increased proliferation, while non-responders had higher zinc metabolism programs before CRT. Longitudinally, we found large expansions of myeloid cells during CRT and persistent inflammation in the worse responders. Additionally, the upregulation of gastric subtype expression programs on cancer cells during CRT was strongly associated with non-responders. Collectively, this study provides insights into the biology of CRT responses and identifies novel biomarkers and actionable targets to enhance CRT efficacy in patients.
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| Overall design |
We used scRNA-seq to profile 83 longitudinal samples from Esophageal adenocarcinoma patients that were treated with chemoradiation (CRT) to dilineate the CRT resistance mechanism.
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| Contributor(s) |
Ye R, Navin N, Lin S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 16, 2024 |
| Last update date |
Oct 16, 2024 |
| Contact name |
Nicholas Navin |
| E-mail(s) |
NNavin@mdanderson.org, AKCasasent@mdanderson.org
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| Organization name |
MD anderson Cancer Center
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| Department |
Systems Biology
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| Lab |
Navin Lab
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| Street address |
6565 MD Anderson Blvd
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| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (2) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
| GPL30173 |
NextSeq 2000 (Homo sapiens) |
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| Samples (83)
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| Relations |
| BioProject |
PRJNA1100939 |
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