NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE263983 Query DataSets for GSE263983
Status Public on May 30, 2024
Title Molecular insights into female hybrid sterility in interspecific crosses between Drosophila melanogaster and Drosophila simulans (small RNA-Seq)
Organisms Drosophila melanogaster; Drosophila simulans; Drosophila melanogaster x Drosophila simulans
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Species of the genus Drosophila have served as favorite models in speciation studies, however genetic factors of the interspecific hybrid sterility are underinvestigated to date. Here we performed the analysis of reproductive incompatibilities of hybrid females in crossing Drosophila melanogaster females and Drosophila simulans males. Using transcriptomic data analysis, molecular, cellular and genetic approaches we analyzed differential gene expression, transposable element (TE) activity, piRNA biogenesis and functional defects of oogenesis in hybrids. A premature GSC loss was a most prominent defect of oogenesis in hybrid ovaries. Owing differential expression of genes encoding components of the piRNA pathway rhino and deadlock, functional RDCmel complex in hybrid ovaries was not assembled. At the same time the activity of RDCsim complex was maintained in hybrids, independently from the genomic origin of piRNA clusters. Despite identification of a cohort of overexpressed TEs in hybrid ovaries we found no evidences that their activity can be considered as the main cause of hybrid sterility. We revealed complex pattern of Vasa protein expression in hybrid germline, including partial AT-chX piRNA targeting of vasasim allele and significant developmental delay of vasamel expression. We came to the conclusions that complex multi-locus genetic changes between the species were responsible for hybrid sterility phenotype.
 
Overall design To evaluate piRNA clusters activity and TE-related piRNA level in ovaries of interspecific hybrids and parental species small RNA libraries were created.
 
Contributor(s) Kotov AA
Citation(s) 38891872
Submission date Apr 15, 2024
Last update date Jun 28, 2024
Contact name Alexei A. Kotov
E-mail(s) kotov_alexei@mail.ru
Organization name Koltzov Institute of Developmental Biology of the Russian Academy of Sciences
Street address 26 Vavilov Street
City Moscow
ZIP/Postal code 119334
Country Russia
 
Platforms (3)
GPL25244 Illumina NovaSeq 6000 (Drosophila melanogaster)
GPL33169 Illumina NovaSeq 6000 (Drosophila simulans)
GPL34386 Illumina NovaSeq 6000 (Drosophila melanogaster x Drosophila simulans)
Samples (6)
GSM8208285 D. melanogaster hmr2, ovaries, small RNA rep1
GSM8208286 D. melanogaster hmr2, ovaries, small RNA rep2
GSM8208287 hybrids D. Melanogaster x D.simulans, ovaries, small RNA rep1
This SubSeries is part of SuperSeries:
GSE263985 Molecular insights into female hybrid sterility in interspecific crosses between Drosophila melanogaster and Drosophila simulans
Relations
BioProject PRJNA1100504

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE263983_TEset_bowtie_mappers_counts.csv.gz 1.7 Kb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap