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| Status |
Public on May 30, 2024 |
| Title |
Molecular insights into female hybrid sterility in interspecific crosses between Drosophila melanogaster and Drosophila simulans (RNA-Seq) |
| Organisms |
Drosophila melanogaster; Drosophila simulans; Drosophila melanogaster x Drosophila simulans |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Species of the genus Drosophila have served as favorite models in speciation studies, however genetic factors of the interspecific hybrid sterility are underinvestigated to date. Here we performed the analysis of reproductive incompatibilities of hybrid females in crossing Drosophila melanogaster females and Drosophila simulans males. Using transcriptomic data analysis, molecular, cellular and genetic approaches we analyzed differential gene expression, transposable element (TE) activity, piRNA biogenesis and functional defects of oogenesis in hybrids. A premature GSC loss was a most prominent defect of oogenesis in hybrid ovaries. Owing differential expression of genes encoding components of the piRNA pathway rhino and deadlock, functional RDCmel complex in hybrid ovaries was not assembled. At the same time the activity of RDCsim complex was maintained in hybrids, independently from the genomic origin of piRNA clusters. Despite identification of a cohort of overexpressed TEs in hybrid ovaries we found no evidences that their activity can be considered as the main cause of hybrid sterility. We revealed complex pattern of Vasa protein expression in hybrid germline, including partial AT-chX piRNA targeting of vasasim allele and significant developmental delay of vasamel expression. We came to the conclusions that complex multi-locus genetic changes between the species were responsible for hybrid sterility phenotype.
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| Overall design |
To analyze the nature of hybrid sterility in crossings between D. Melanogaster (hmr2) and D. Simulans (w501) flies we prepared, sequenced, and analyzed whole-transcriptome libraries of mRNA isolated from the ovaries of interspecific hybrids and parental species.
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| Contributor(s) |
Kotov AA |
| Citation(s) |
38891872 |
| |
| Submission date |
Apr 15, 2024 |
| Last update date |
Jun 28, 2024 |
| Contact name |
Alexei A. Kotov |
| E-mail(s) |
kotov_alexei@mail.ru
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| Organization name |
Koltzov Institute of Developmental Biology of the Russian Academy of Sciences
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| Street address |
26 Vavilov Street
|
| City |
Moscow |
| ZIP/Postal code |
119334 |
| Country |
Russia |
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| Platforms (3) |
| GPL25244 |
Illumina NovaSeq 6000 (Drosophila melanogaster) |
| GPL33169 |
Illumina NovaSeq 6000 (Drosophila simulans) |
| GPL34386 |
Illumina NovaSeq 6000 (Drosophila melanogaster x Drosophila simulans) |
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| Samples (6)
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| GSM8208279 |
D. melanogaster hmr2, ovaries, rep1 |
| GSM8208280 |
D. melanogaster hmr2, ovaries, rep2 |
| GSM8208281 |
hybrids D. Melanogaster x D.simulans, ovaries, rep1 |
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| This SubSeries is part of SuperSeries: |
| GSE263985 |
Molecular insights into female hybrid sterility in interspecific crosses between Drosophila melanogaster and Drosophila simulans |
|
| Relations |
| BioProject |
PRJNA1100501 |
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