 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jul 31, 2024 |
| Title |
Molecular mechanisms of Target organ damage in youth with primary hypertension [bulk RNA-Seq 3] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
High blood pressure or Primary Hypertension is associated with target organ damage (TOD) in both adults and youth, where TOD predicts the adverse cardiovscular (CV) events. The mechanisms involved in blood pressure mediated TOD are limitedly understood in onset of TOD. To understand this, first using RNA sequencing, we identified candidate differentially expressed genes in circulating PBMCs of study participants with and without hypertension and elevated left ventrivlar mass index . Next, through miRNA and whole genome bisulphite sequencing of circulating PBMCs we determined the epigenetic regulators of TOD. Also, using LCMS, serum proteomics, the key circulatory protein involved in advancing CV- TOD events was identified. In conclusion, multiomics approach was utilized to define the circulatory regulators involved in BP mediated CV-TOD.
|
| |
|
| Overall design |
The design and methods of the SHIP AHOY (Study of High Blood Pressure in Pediatrics, Adult Hypertension Onset in Youth) project have been discussed elsewhere (Mendizabal B, et al., 2018, Hypertension . 2018 Sep;72(3):625-631.). Briefly, in the current cross-sectional study, youth aged 11 to less than 19 years with BP levels (N=397) across the BP distribution, were enrolled at five clinical sites (Cincinnati, Houston, Philadelphia, Rochester, and Seattle) from 2015 to 2017 from pediatric specialty clinics (four nephrology clinics, and one joint nephrology and cardiology clinic). Efforts were made to achieve body mass index (BMI) balance across BP groups by periodically checking differences in BMI percentile among groups and actively recruiting normotensive obese controls. Out of 397 subjects, 132 adolescents of both sexes and all races with normal (Cut off: SBP <80th%), and high- systolic blood pressure (Cut off: SBP >90th%), and then stratified by with or without TOD, like Low LVMI (Left ventricular mass index) (Cut off: <38.6 gm/m2.7), and High LVMI (Cut off: >38.6 gm/m2.7), were selected in the present study cohort. Urine and Blood samples (for PBNCs and serum) were collected to investigate genetic, epigenetic, and proteomics changes that influence the development of hypertensive TOD in youth. We excluded youth with symptomatic severe hypertension, on antihypertensive or lipid-lowering medication in the past 6 months, with diabetes mellitus (type 1 or 2), kidney disease, or other chronic medical conditions. All study participants and their parents provided written informed consent and assent according to local investigational review board requirements.
|
| |
|
| Contributor(s) |
Brady TM, Ananthamohan K, Arif M, Daniels S, Falkner B, Ferguson M, Flynn JT, Hanevold C, Hooper S, Inglefinger J, Lande M, Martin LJ, SK M, Mitsnefes M, Rosner B, Samuels JA, Kuffel G, Zilliox MJ, Becker RC, Urbina EM, Sadayappan S |
| Citation(s) |
38948714 |
| |
| Submission date |
Mar 29, 2024 |
| Last update date |
Jul 31, 2024 |
| Contact name |
Kwangmin Choi |
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Department |
Experimental Hematology & Cancer Biology
|
| Street address |
333 Burnet Avenue
|
| City |
Cincinnati |
| State/province |
Ohio |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (20)
|
| GSM8179760 |
PBMC RNA, repeat, Low Blood pressure with Low LVMI , 1 |
| GSM8179761 |
PBMC RNA, repeat, Low Blood pressure with Low LVMI , 2 |
| GSM8179762 |
PBMC RNA, repeat, Low Blood pressure with Low LVMI , 3 |
| GSM8179763 |
PBMC RNA, repeat, Low Blood pressure with Low LVMI , 4 |
| GSM8179764 |
PBMC RNA, repeat, Low Blood pressure with Low LVMI , 5 |
| GSM8179765 |
PBMC RNA, repeat, Low Blood pressure with Low LVMI , 6 |
| GSM8179766 |
PBMC RNA, repeat, Low Blood pressure with Low LVMI , 7 |
| GSM8179767 |
PBMC RNA, repeat, Low Blood pressure with Low LVMI , 8 |
| GSM8179768 |
PBMC RNA, repeat, Low Blood pressure with Low LVMI , 9 |
| GSM8179769 |
PBMC RNA, repeat, Low Blood pressure with Low LVMI , 10 |
| GSM8179770 |
PBMC RNA, repeat, High Blood pressure with High LVMI , 1 |
| GSM8179771 |
PBMC RNA, repeat, High Blood pressure with High LVMI , 2 |
| GSM8179772 |
PBMC RNA, repeat, High Blood pressure with High LVMI , 3 |
| GSM8179773 |
PBMC RNA, repeat, High Blood pressure with High LVMI , 4 |
| GSM8179774 |
PBMC RNA, repeat, High Blood pressure with High LVMI , 5 |
| GSM8179775 |
PBMC RNA, repeat, High Blood pressure with High LVMI , 6 |
| GSM8179776 |
PBMC RNA, repeat, High Blood pressure with High LVMI , 7 |
| GSM8179777 |
PBMC RNA, repeat, High Blood pressure with High LVMI , 8 |
| GSM8179778 |
PBMC RNA, repeat, High Blood pressure with High LVMI , 9 |
| GSM8179779 |
PBMC RNA, repeat, High Blood pressure with High LVMI , 10 |
|
| This SubSeries is part of SuperSeries: |
| GSE262821 |
Molecular mechanisms of Target organ damage in youth with primary hypertension |
|
| Relations |
| BioProject |
PRJNA1093431 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE262815_Becker_Normalized_Counts.xlsx |
5.9 Mb |
(ftp)(http) |
XLSX |
| GSE262815_Sakthi_AllGenes_Expression_3-27-18.xlsx |
1.6 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
|
|
|
|
 |
Less...
More...