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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Sep 06, 2024 |
| Title |
Non-homogenous intratumor ionizing radiation doses synergize with PD1 and CXCR2 blockade |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Partial tumor irradiation combining low dose radiation with high dose radiation within the same tumor mass would prompt an antitumor effect when combined with immunomodulators. Partial irradiations (50%/50%) of subcutaneous MC38 tumors with millimetric precision to deliver low dose radiation (2Gy) and high dose radiation (16Gy) within the same tumor was performed and single-cell RNA sequencing of CD45+ cells analysis was done in both the two part of the tumor to identify tumor-infiltrating immune cells.
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| Overall design |
scRNA-seq datas of CD45+ cells from subcutaneous MC38 tumors from control mice (Ctrl, 4 replicates, total tumor irradiation (TI) at 2 Gy (TI2, 2 replicates) or 16 Gy (TI16, 2 replicates) or partial irradiations (PI) (50%/50%) either 16Gy/0Gy (PI16/0, 2 replicates) or 16Gy/2Gy (PI16/2, 2 replicates). A total of 16 samples is included in this study.
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| Contributor(s) |
Milliat F, Mondini M, Bergeron P, Benadjaoud A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Mar 28, 2024 |
| Last update date |
Sep 07, 2024 |
| Contact name |
Fabien Milliat |
| E-mail(s) |
fabien.milliat@irsn.fr
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| Phone |
0630963079
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| Organization name |
IRSN
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| Lab |
LRMed
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| Street address |
31 avenue de la division Leclerc
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| City |
Fontenay-Aux-Roses |
| ZIP/Postal code |
92262 |
| Country |
France |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (16)
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| GSM8174006 |
CD45+ of Control tumor mice 4 |
| GSM8174007 |
CD45+ of total tumor volume at 2Gy, mice 1 |
| GSM8174008 |
CD45+ of total tumor volume at 2Gy, mice 2 |
| GSM8174009 |
CD45+ of total tumor volume at 16Gy, mice 1 |
| GSM8174010 |
CD45+ of total tumor volume at 16Gy, mice 2 |
| GSM8174011 |
CD45+ of partial tumor volume 16/0 Gy RT (PI16/0), 0GY part , mice 1 |
| GSM8174012 |
CD45+ of partial tumor volume 16/0 Gy RT (PI16/0), 0GY part , mice 2 |
| GSM8174013 |
CD45+ of partial tumor volume 16/0 Gy RT (PI16/0), 16GY part , mice 1 |
| GSM8174014 |
CD45+ of partial tumor volume 16/0 Gy RT (PI16/0), 16GY part , mice 2 |
| GSM8174015 |
CD45+ of partial tumor volume 16/2 Gy RT (PI16/2), 2GY part , mice 1 |
| GSM8174016 |
CD45+ of partial tumor volume 16/2 Gy RT (PI16/2), 2GY part , mice 2 |
| GSM8174017 |
CD45+ of partial tumor volume 16/2 Gy RT (PI16/2), 16GY part , mice 1 |
| GSM8174018 |
CD45+ of partial tumor volume 16/2 Gy RT (PI16/2), 16GY part , mice 2 |
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| Relations |
| BioProject |
PRJNA1093027 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE262699_RAW.tar |
1.3 Gb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
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