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| Status |
Public on Jul 03, 2024 |
| Title |
Gene expression changes in SHI-1 cells following exposure to disulfiram/copper, niclosamde or combined drugs |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Disulfiram and niclosamide were identified as drugs that induced depletion of an MLL-AF9-luciferase fusion protein in THP-1 AML cells, in a bioluminescence sreen. MLL-fusion protein depletion was confirmed in AML and ALL cell lines expressing different MLL-fusion proteins. Combination of disulfiram with niclosamide was found to enhance depletion of MLL-fusion proteins. To investigate whether this enhanced depletion resulted in increased suppression of downstream target genes. MLL-AF6 expressing SHI-1 cells were exposed to disulfiram/copper, nciclosamide or combined drugs. SHI-1 cells were treated for 16 hours with 0.3uM disulfiram / 1uM copper, 5uM niclosamide or combined drugs and RNAseq performed on isolated RNA.
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| Overall design |
Total cellular RNA was purified from SHI-1 cells treated with DMSO, 0.3uM disulfiram / 1uM copper, 5uM niclosamide or combined drugs for 16 hours, from three independent experiments
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| Contributor(s) |
Williams O, de Boer J, Che N, Cantilena S |
| Citation(s) |
39152521 |
| |
| Submission date |
Mar 28, 2024 |
| Last update date |
Sep 11, 2024 |
| Contact name |
Owen Williams |
| E-mail(s) |
owen.williams@ucl.ac.uk
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| Phone |
+4420790542180
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| Organization name |
UCL GOS ICH
|
| Department |
Cancer section
|
| Street address |
30 Guilford Street
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| City |
London |
| ZIP/Postal code |
WC1N 1EH |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (12)
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| GSM8173506 |
SHI-1 0.3uM 1 disulfiram / 1uM copper 16 hours |
| GSM8173507 |
SHI-1 0.3uM 2 disulfiram / 1uM copper 16 hours |
| GSM8173508 |
SHI-1 0.3uM 3 disulfiram / 1uM copper 16 hours |
| GSM8173509 |
SHI-1 5uM 1 niclosamide 16 hours |
| GSM8173510 |
SHI-1 5uM 2 niclosamide 16 hours |
| GSM8173511 |
SHI-1 5uM 3 niclosamide 16 hours |
| GSM8173512 |
SHI-1 5uM 1 niclosamide and 0.3uM disulfiram / 1uM copper 16 hours |
| GSM8173513 |
SHI-1 5uM 2 niclosamide and 0.3uM disulfiram / 1uM copper 16 hours |
| GSM8173514 |
SHI-1 5uM 3 niclosamide and 0.3uM disulfiram / 1uM copper 16 hours |
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| Relations |
| BioProject |
PRJNA1092955 |
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