|
| Status |
Public on Jun 20, 2024 |
| Title |
The role of inflammatory macrophages in pathophysiology of experimental autoimmune myocarditis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Myocarditis is an inflammatory disease of the cardiac muscle characterized by an influx of inflammatory cells, predominantly of myeloid lineage. The progression of myocarditis to a dilated cardiomyopathy phenotype and heart failure is markedly influenced by TGF-β signalling. The aim of this study was to investigate the role of TGF-β signalling in inflammatory cardiac macrophages in the development of myocarditis and post-inflammatory fibrosis. Experimental autoimmune myocarditis (EAM) was induced in the LysM-Cre x R26-stop-EYFP x Tgfbr2-fl/fl transgenic mice showing impaired TGF-β signalling in the myeloid lineage and the LysM-Cre x R26-stop-EYFP control mice. Inflammatory macrophages were sorted from the inflamed hearts and analyzed for differential gene expression using whole genome transcriptomics.
|
| |
|
| Overall design |
To investigate the role of TGF-β signalling in inflammatory cardiac macrophages in the development of myocarditis and post-inflammatory fibrosis we used LysM-Cre x R26-stop-EYFP x Tgfbr2-fl/fl transgenic mice showing impaired TGF-β signalling in the myeloid lineage and the LysM-Cre x R26-stop-EYFP control mice. We induced myocarditis and collected inflamed heart. Sorted inflammatory cardiac macrophages were used for whole genome transcriptomics.
|
| |
|
| Contributor(s) |
Tkacz K, Błyszczuk P |
| Citation(s) |
38891767 |
| |
| Submission date |
Mar 27, 2024 |
| Last update date |
Jun 21, 2024 |
| Contact name |
Karolina Tkacz |
| E-mail(s) |
karolina.tkacz93@gmail.com
|
| Organization name |
Jagiellonian University Medical College
|
| Street address |
ul. Wielicka 256
|
| City |
Cracow |
| ZIP/Postal code |
30-663 |
| Country |
Poland |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (8)
|
| GSM8173190 |
LysM-Cre x R26-stop-EYFP inflammatory cardiac macrophages biol rep 1 |
| GSM8173191 |
LysM-Cre x R26-stop-EYFP inflammatory cardiac macrophages biol rep 2 |
| GSM8173192 |
LysM-Cre x R26-stop-EYFP inflammatory cardiac macrophages biol rep 3 |
| GSM8173193 |
LysM-Cre x R26-stop-EYFP inflammatory cardiac macrophages biol rep 4 |
| GSM8173194 |
LysM-Cre x R26-stop-EYFP x Tgfbr2-fl/fl inflammatory cardiac macrophages biol rep 1 |
| GSM8173195 |
LysM-Cre x R26-stop-EYFP x Tgfbr2-fl/fl inflammatory cardiac macrophages biol rep 2 |
| GSM8173196 |
LysM-Cre x R26-stop-EYFP x Tgfbr2-fl/fl inflammatory cardiac macrophages biol rep 3 |
| GSM8173197 |
LysM-Cre x R26-stop-EYFP x Tgfbr2-fl/fl inflammatory cardiac macrophages biol rep 4 |
|
| Relations |
| BioProject |
PRJNA1092732 |
Less...
More...