|
| Status |
Public on May 30, 2024 |
| Title |
BCAT1 contributes to the developmentproliferation and leukemogenesis of TKI-resistant CML |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this report, we revealed that branched chain amino acid transaminase 1 (BCAT1) is highly enriched in both mouse and human TKI-resistant CML cells. Leukemia was almost completely abrogated upon BCAT1 knockdown during transplantation in a BCR-ABLT315I-induced murine TKI-resistant CML model . Moreover, knockdown of BCAT1 led to a dramatic decrease in the proliferation of TKI-resistant human leukemia cell lines. BCAA/BCAT1 signaling enhanced the phosphorylation of CREB, which is required for maintenance of TKI-resistant CML cells. Importantly, blockade of BCAA/BCAT1 signaling efficiently inhibited leukemogenesis both in vivo and in vitro.
|
| |
|
| Overall design |
We performed transcriptome sequencing (RNA-seq) using BCAT1-knockdown and Scramble 32Dcl3 cells
|
| |
|
| Contributor(s) |
Jiang Y, Huang D |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Mar 25, 2024 |
| Last update date |
May 30, 2024 |
| Contact name |
yu Jiang |
| E-mail(s) |
jiangyu1995@sjtu.edu.cn
|
| Phone |
+8615213086391
|
| Organization name |
Shanghai Jiao Tong University School of Medicine
|
| Department |
Faculty of Basic Medicine
|
| Lab |
Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education
|
| Street address |
280 Chongqing South Road, Shanghai
|
| City |
Shanghai |
| ZIP/Postal code |
200025 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA1091685 |
