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| Status |
Public on Aug 31, 2024 |
| Title |
Reproductive biomarker response to PFAS-contaminated groundwater [liver] |
| Organism |
Pimephales promelas |
| Experiment type |
Expression profiling by array
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| Summary |
The use of aqueous film-forming foams (AFFF) at fire-training areas (FTAs) has introduced into ground- and surface waters a complex mixture of per- and poly-fluorinated alkyl substances (PFAS). The toxicity of environmental PFAS mixtures to wildlife is not well understood and presents a knowledge gap that limits accurate risk assessment. To evaluate reproductive biomarker responses to complex environmental PFAS mixtures, we conducted a series of on-site experiments using flow-through mobile laboratories exposing fish to groundwater impacted by a legacy FTA and an adjacent reference site
A 60K fathead minnow microarray was used to quantify gene expression patterns in the testis and liver of fish exposed to water from Fire Training Area 1 and 2 relative to a reference site.
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| Overall design |
Fathead minnows were exposed to ground water in a mobile laboratory at the fire training areas. Sample sizes included the following: Reference site (n=6), Control effluent (n=8), FTA-1 (n=5), FTA-2 (n=6) and FTA-2/Ref (50/50 mix) (n=6).
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| Contributor(s) |
Vajda AM, Jenkins JA, Bertolatus DW, Keefe SH, LeBlanc DR, Dethloff AR, Martyniuk CJ, Miller CS, Lohman R, Barber LB |
| Citation missing |
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| |
| Submission date |
Mar 14, 2024 |
| Last update date |
Aug 31, 2024 |
| Contact name |
Christopher Martyniuk |
| E-mail(s) |
cmartyn@ufl.edu
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| Organization name |
University of Florida
|
| Department |
Physiological Sciences
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| Lab |
Martyniuk
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| Street address |
2063 Mowry
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| City |
Gainesville |
| State/province |
Florida |
| ZIP/Postal code |
32608 |
| Country |
USA |
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| Platforms (1) |
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| Samples (31)
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| Relations |
| BioProject |
PRJNA1088243 |
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