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| Status |
Public on May 17, 2024 |
| Title |
Compare transcriptome profile of reference strain and mutant of HapX during A.niger grown on maltose |
| Organism |
Aspergillus niger |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Transcriptome analysis was performed to identify the differentially expressed genes between hapX mutant and reference strains during their growth on maltose
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| Overall design |
The strains were pre-grown in complete medium containing 2% (w/v) fructose for 16h. The mycelium was harvested and washed with minimal medium (MM) , and then was transferred to 50 ml MM (pH 6) with 25 mM maltose. After 2h of incubation, the mycelium was harvested and frozen in liquid nitrogen. RNA was extracted from grinded mycelia and were single-end sequenced using Illumina HiSeqTM 2000 platform . The reads were mapped to the genome of Aspergillus niger NRRL3 (https://mycocosm.jgi.doe.gov/Aspni_NRRL3_1),
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| Contributor(s) |
Kowalczyk JE, Peng M, de Vries RP |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 29, 2024 |
| Last update date |
May 18, 2024 |
| Contact name |
Ronald de vries |
| E-mail(s) |
fungalphysiology@gmail.com
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| Phone |
+31(0)30-2122600
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| Organization name |
westerdijkinstitute
|
| Street address |
Uppsalalaan 8
|
| City |
Utrecht |
| State/province |
Utrecht |
| ZIP/Postal code |
3584 CT |
| Country |
Netherlands |
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| Platforms (1) |
| GPL21721 |
Illumina HiSeq 2000 (Aspergillus niger) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA1082370 |
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